Application of holistic liquid chromatography-high resolution mass spectrometry based urinary metabolomics for prostate cancer detection and biomarker discovery

Tong Zhang; David G Watson; Lijie Wang; Muhammad Abbas; Laura Murdoch; Lisa Bashford; Imran Ahmad; Nga-Yee Lam; Anthony C F Ng; Hing Y Leung

doi:10.1371/journal.pone.0065880

Application of holistic liquid chromatography-high resolution mass spectrometry based urinary metabolomics for prostate cancer detection and biomarker discovery

Tong Zhang, David G Watson, Lijie Wang, Muhammad Abbas, Laura Murdoch, Lisa Bashford, Imran Ahmad, Nga-Yee Lam, Anthony C F Ng, Hing Y Leung

Research output: Contribution to journal › Article › peer-review

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Abstract

Human exhibit wide variations in their metabolic profiles because of differences in genetic factors, diet and lifestyle. Therefore in order to detect metabolic differences between individuals robust analytical methods are required. A protocol was produced based on the use of Liquid Chromatography- High Resolution Mass Spectrometry (LC-HRMS) in combination with orthogonal Hydrophilic Interaction (HILIC) and Reversed Phase (RP) liquid chromatography methods for the analysis of the urinary metabolome, which was then evaluated as a diagnostic tool for prostate cancer (a common but highly heterogeneous condition). The LC-HRMS method was found to be robust and exhibited excellent repeatability for retention times (0.9. In addition, using the receiver operator characteristics (ROC) test, the area under curve (AUC) for the combination of the four best characterised biomarker compounds was 0.896. The four biomarker compounds were also found to differ significantly (P

Original language	English
Pages (from-to)	e65880
Journal	PLoS ONE
Volume	8
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0065880
Publication status	Published - 18 Jun 2013

Keywords

holistic
liquid chromatography-high resolution mass spectrometry
urinary metabolomics
prostate cancer detection
biomarker discovery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1371/journal.pone.0065880

ZhangWatsonWangAbbas-etal-PLoSOne2013Final published version, 782 KBLicence: CC BY 4.0

Cite this

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AU - Watson, David G

AU - Wang, Lijie

AU - Abbas, Muhammad

AU - Murdoch, Laura

AU - Bashford, Lisa

AU - Ahmad, Imran

AU - Lam, Nga-Yee

AU - Ng, Anthony C F

AU - Leung, Hing Y

PY - 2013/6/18

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N2 - Human exhibit wide variations in their metabolic profiles because of differences in genetic factors, diet and lifestyle. Therefore in order to detect metabolic differences between individuals robust analytical methods are required. A protocol was produced based on the use of Liquid Chromatography- High Resolution Mass Spectrometry (LC-HRMS) in combination with orthogonal Hydrophilic Interaction (HILIC) and Reversed Phase (RP) liquid chromatography methods for the analysis of the urinary metabolome, which was then evaluated as a diagnostic tool for prostate cancer (a common but highly heterogeneous condition). The LC-HRMS method was found to be robust and exhibited excellent repeatability for retention times (0.9. In addition, using the receiver operator characteristics (ROC) test, the area under curve (AUC) for the combination of the four best characterised biomarker compounds was 0.896. The four biomarker compounds were also found to differ significantly (P

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Application of holistic liquid chromatography-high resolution mass spectrometry based urinary metabolomics for prostate cancer detection and biomarker discovery

Abstract

Keywords

UN SDGs

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