Apoptotic activity of new oxisterigmatocystin derivatives from the marine-derived fungus Aspergillus nomius NC06

Muh. Ade Artasasta, Yanwirasti Yanwirasti, Muhammad Taher, Akmal Djamaan, Ni Putu Ariantari, Ru Angelie Edrada-Ebel, Dian Handayani

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Abstract

Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. Aspergillus nomius NC06 was isolated from the marine sponge Neopetrosia chaliniformis. This fungus was cultured on rice medium and yielded four compounds including three new oxisterigmatocystins, namely, J, K, and L (1, 2, and 3), and one known compound, aspergillicin A (4). Structures of the compounds were elucidated by 1D and 2D NMR spectroscopy and by high-resolution mass spectrometry. The isolated compounds were tested for cytotoxic activity against HT 29 colon cancer cells, where compounds 1, 2, and 4 exhibited IC 50 values of 6.28, 15.14, and 1.63 µM, respectively. Under the fluorescence microscope by using a double staining method, HT 29 cells were observed to be viable, apoptotic, and necrotic after treatment with the cytotoxic compounds 1, 2, and 4. The result shows that compounds 1 and 2 were able to induce apoptosis and cell death in HT 29 cells.

Original languageEnglish
Article number631
Pages (from-to)e631
Number of pages10
JournalMarine Drugs
Volume19
Issue number11
Early online date11 Nov 2021
DOIs
Publication statusPublished - 11 Nov 2021

Keywords

  • marine sponge-derived fungus
  • Neopetrosia chaliniformis
  • Aspergillus nomius
  • oxisterigmatocystin
  • cytotoxic activity
  • colon cancer
  • apoptosis cells

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