Antioxidant properties of amniotic membrane: novel observations from a pilot study

David Lockington, Pankaj Agarwal, David Young, Muriel Caslake, Kanna Ramaesh

Research output: Contribution to journalArticle

8 Citations (Scopus)
118 Downloads (Pure)

Abstract

Objective
Amniotic membrane (AM) is used to manage various debilitated ocular surface conditions. The impact of oxidative stress and free radicals on the ocular surface is increasingly being recognized. Hyaluronic acid (HA) has anti-inflammatory properties and is abundantly present in AM. In this in vitro pilot study, we investigated the potential of AM for intrinsic free radical scavenging properties. Methods
Strips of AM were incubated in sealed tubes with hydrogen peroxide (H2O2). After being sonicated, uptake of reactive oxygen species (ROS) was measured by the Amplex Red Hydrogen Peroxide/Peroxidase assay. For comparison, 1630 kDA HA was used.
Results
There was uptake of ROS by all AM samples, which decreased with increasing concentrations of H2O2. Mean ROS uptake for 5 different AMs at 1 hour was significantly greater for 50 μM (83%; SD 11.7, SEM 5.23) compared with 100 μM (67%; SD 20.48, SEM 9.16; p = 0.028; 95% CI 2.8-29.2). The HA comparison group showed similar uptake and trend.
Conclusion
This pilot study demonstrates that AM is able to remove ROS from its environment. Demonstrating total antioxidant capacity in AM provides evidence for use as a free radical scavenger. The antioxidant properties of AM and the contribution from HA require more research.
Original languageEnglish
Pages (from-to)426-430
Number of pages5
JournalCanadian Journal of Ophthalmology. Journal Canadien d'Ophtalmologie
Volume49
Issue number5
DOIs
Publication statusPublished - 2 Oct 2014

    Fingerprint

Keywords

  • amnion
  • antioxidants
  • free radical scavengers
  • humans
  • hydrogen peroxide
  • oxidants
  • pilot projects
  • reactive oxygen species

Cite this