Antioxidant properties of amniotic membrane: novel observations from a pilot study

David Lockington, Pankaj Agarwal, David Young, Muriel Caslake, Kanna Ramaesh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective
Amniotic membrane (AM) is used to manage various debilitated ocular surface conditions. The impact of oxidative stress and free radicals on the ocular surface is increasingly being recognized. Hyaluronic acid (HA) has anti-inflammatory properties and is abundantly present in AM. In this in vitro pilot study, we investigated the potential of AM for intrinsic free radical scavenging properties. Methods
Strips of AM were incubated in sealed tubes with hydrogen peroxide (H2O2). After being sonicated, uptake of reactive oxygen species (ROS) was measured by the Amplex Red Hydrogen Peroxide/Peroxidase assay. For comparison, 1630 kDA HA was used.
Results
There was uptake of ROS by all AM samples, which decreased with increasing concentrations of H2O2. Mean ROS uptake for 5 different AMs at 1 hour was significantly greater for 50 μM (83%; SD 11.7, SEM 5.23) compared with 100 μM (67%; SD 20.48, SEM 9.16; p = 0.028; 95% CI 2.8-29.2). The HA comparison group showed similar uptake and trend.
Conclusion
This pilot study demonstrates that AM is able to remove ROS from its environment. Demonstrating total antioxidant capacity in AM provides evidence for use as a free radical scavenger. The antioxidant properties of AM and the contribution from HA require more research.
LanguageEnglish
Pages426-430
Number of pages5
JournalCanadian Journal of Ophthalmology. Journal Canadien d'Ophtalmologie
Volume49
Issue number5
DOIs
Publication statusPublished - 2 Oct 2014

Fingerprint

Reactive Oxygen Species
Antioxidants
Amnion
Hyaluronic Acid
Free Radicals
Membrane
Hydrogen Peroxide
Oxidative Stress
Peroxidase
Free Radical Scavengers
Tube
Anti-Inflammatory Agents
Membranes
Observation
Research
Demonstrate

Keywords

  • amnion
  • antioxidants
  • free radical scavengers
  • humans
  • hydrogen peroxide
  • oxidants
  • pilot projects
  • reactive oxygen species

Cite this

Lockington, David ; Agarwal, Pankaj ; Young, David ; Caslake, Muriel ; Ramaesh, Kanna. / Antioxidant properties of amniotic membrane : novel observations from a pilot study. In: Canadian Journal of Ophthalmology. Journal Canadien d'Ophtalmologie. 2014 ; Vol. 49, No. 5. pp. 426-430.
@article{3ebb74b8b62647dcb5320a761d41cc1b,
title = "Antioxidant properties of amniotic membrane: novel observations from a pilot study",
abstract = "Objective Amniotic membrane (AM) is used to manage various debilitated ocular surface conditions. The impact of oxidative stress and free radicals on the ocular surface is increasingly being recognized. Hyaluronic acid (HA) has anti-inflammatory properties and is abundantly present in AM. In this in vitro pilot study, we investigated the potential of AM for intrinsic free radical scavenging properties. Methods Strips of AM were incubated in sealed tubes with hydrogen peroxide (H2O2). After being sonicated, uptake of reactive oxygen species (ROS) was measured by the Amplex Red Hydrogen Peroxide/Peroxidase assay. For comparison, 1630 kDA HA was used.ResultsThere was uptake of ROS by all AM samples, which decreased with increasing concentrations of H2O2. Mean ROS uptake for 5 different AMs at 1 hour was significantly greater for 50 μM (83{\%}; SD 11.7, SEM 5.23) compared with 100 μM (67{\%}; SD 20.48, SEM 9.16; p = 0.028; 95{\%} CI 2.8-29.2). The HA comparison group showed similar uptake and trend. ConclusionThis pilot study demonstrates that AM is able to remove ROS from its environment. Demonstrating total antioxidant capacity in AM provides evidence for use as a free radical scavenger. The antioxidant properties of AM and the contribution from HA require more research.",
keywords = "amnion, antioxidants, free radical scavengers, humans, hydrogen peroxide, oxidants, pilot projects, reactive oxygen species",
author = "David Lockington and Pankaj Agarwal and David Young and Muriel Caslake and Kanna Ramaesh",
note = "Copyright {\circledC} 2014 Canadian Ophthalmological Society. Published by Elsevier Inc. All rights reserved.",
year = "2014",
month = "10",
day = "2",
doi = "10.1016/j.jcjo.2014.07.005",
language = "English",
volume = "49",
pages = "426--430",
journal = "Canadian Journal of Ophthalmology. Journal Canadien d'Ophtalmologie",
issn = "0008-4182",
publisher = "Canadian Ophthalmological Society",
number = "5",

}

Antioxidant properties of amniotic membrane : novel observations from a pilot study. / Lockington, David; Agarwal, Pankaj; Young, David; Caslake, Muriel; Ramaesh, Kanna.

In: Canadian Journal of Ophthalmology. Journal Canadien d'Ophtalmologie, Vol. 49, No. 5, 02.10.2014, p. 426-430.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant properties of amniotic membrane

T2 - Canadian Journal of Ophthalmology. Journal Canadien d'Ophtalmologie

AU - Lockington, David

AU - Agarwal, Pankaj

AU - Young, David

AU - Caslake, Muriel

AU - Ramaesh, Kanna

N1 - Copyright © 2014 Canadian Ophthalmological Society. Published by Elsevier Inc. All rights reserved.

PY - 2014/10/2

Y1 - 2014/10/2

N2 - Objective Amniotic membrane (AM) is used to manage various debilitated ocular surface conditions. The impact of oxidative stress and free radicals on the ocular surface is increasingly being recognized. Hyaluronic acid (HA) has anti-inflammatory properties and is abundantly present in AM. In this in vitro pilot study, we investigated the potential of AM for intrinsic free radical scavenging properties. Methods Strips of AM were incubated in sealed tubes with hydrogen peroxide (H2O2). After being sonicated, uptake of reactive oxygen species (ROS) was measured by the Amplex Red Hydrogen Peroxide/Peroxidase assay. For comparison, 1630 kDA HA was used.ResultsThere was uptake of ROS by all AM samples, which decreased with increasing concentrations of H2O2. Mean ROS uptake for 5 different AMs at 1 hour was significantly greater for 50 μM (83%; SD 11.7, SEM 5.23) compared with 100 μM (67%; SD 20.48, SEM 9.16; p = 0.028; 95% CI 2.8-29.2). The HA comparison group showed similar uptake and trend. ConclusionThis pilot study demonstrates that AM is able to remove ROS from its environment. Demonstrating total antioxidant capacity in AM provides evidence for use as a free radical scavenger. The antioxidant properties of AM and the contribution from HA require more research.

AB - Objective Amniotic membrane (AM) is used to manage various debilitated ocular surface conditions. The impact of oxidative stress and free radicals on the ocular surface is increasingly being recognized. Hyaluronic acid (HA) has anti-inflammatory properties and is abundantly present in AM. In this in vitro pilot study, we investigated the potential of AM for intrinsic free radical scavenging properties. Methods Strips of AM were incubated in sealed tubes with hydrogen peroxide (H2O2). After being sonicated, uptake of reactive oxygen species (ROS) was measured by the Amplex Red Hydrogen Peroxide/Peroxidase assay. For comparison, 1630 kDA HA was used.ResultsThere was uptake of ROS by all AM samples, which decreased with increasing concentrations of H2O2. Mean ROS uptake for 5 different AMs at 1 hour was significantly greater for 50 μM (83%; SD 11.7, SEM 5.23) compared with 100 μM (67%; SD 20.48, SEM 9.16; p = 0.028; 95% CI 2.8-29.2). The HA comparison group showed similar uptake and trend. ConclusionThis pilot study demonstrates that AM is able to remove ROS from its environment. Demonstrating total antioxidant capacity in AM provides evidence for use as a free radical scavenger. The antioxidant properties of AM and the contribution from HA require more research.

KW - amnion

KW - antioxidants

KW - free radical scavengers

KW - humans

KW - hydrogen peroxide

KW - oxidants

KW - pilot projects

KW - reactive oxygen species

UR - http://www.sciencedirect.com/science/article/pii/S0008418214002531

UR - http://www.sciencedirect.com/science/journal/00084182

U2 - 10.1016/j.jcjo.2014.07.005

DO - 10.1016/j.jcjo.2014.07.005

M3 - Article

VL - 49

SP - 426

EP - 430

JO - Canadian Journal of Ophthalmology. Journal Canadien d'Ophtalmologie

JF - Canadian Journal of Ophthalmology. Journal Canadien d'Ophtalmologie

SN - 0008-4182

IS - 5

ER -