Antimicrobial properties of enzymatically triggered self-assembling aromatic peptide amphiphiles

Meghan Hughes, Sisir Debnath, Charles Knapp, Rein Ulijn

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The combination of catalysis and self-assembly influences many key processes in living systems. Synthetic analogues of such systems may provide opportunities to direct biological processes. Previously, it has been demonstrated that enzyme triggered assembly of peptide derivatives can influence bacterial cell death by intracellular fibre formation. In this article, we discuss the self-assembly of 9-fluorenylmethyloxycarbonyl (Fmoc) protected dipeptide amphiphiles, FY, YT, YS, YN and YQ, designing phosphorylated precursors to be alkaline phosphatase responsive. We use microscopy techniques, fluorescence and FTIR to demonstrate differences in molecular assembly and nanoscale architecture in vitro – indicating fibre formation of FY, YT, YS and YN, and spherical self-assembled structures of YQ. As the enzyme is naturally occurring in E. coli, we manipulate conditions to over-express the enzyme and demonstrate the conversion of precursors to self-assembling aromatic peptide amphiphiles in vivo. Furthermore, we test whether antimicrobial activity can be differentially controlled by the introduction of varying aromatic peptide amphiphiles, with the results indicating a similar antimicrobial response for each treatment.
Original languageEnglish
Pages (from-to)1138-1142
Number of pages5
JournalBiomaterials Science
Volume1
Issue number11
DOIs
Publication statusPublished - 26 Jul 2013

Keywords

  • biological processes
  • antimicrobial activity
  • peptide amphiphiles

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