Methanol extracts of the roots of B. brevissima and B. parkeriana were examined for antidiabetic and antimicrobial activities. Six compounds i.e berberine, 8-oxo-berberine, dehydrocheilanthifoline, columbamine, jatrorrhizine and glutamic acid were isolated from the active fractions of these species for the first time. The antidiabetic activity was determined against Protein Tyrosine Phosphatase 1B (PTP 1B), a negative insulin regulator while the antimicrobial activity was performed against four bacterial strains: Mycobacterium marinum ATCC BAA535, sporadic Methicillin Resistant Staphylococcus aureus (SMRSA), endemic Methicillin Resistant Staphylococcus aureus (EMRSA) and Escherichia coli ATCC.8739 at different concentrations. Amongst the isolated compounds 8-oxo-berberine was found to be the most active antidiabetic (29 % of the positive control) while jatrorrhizine was the most active and selective antimicrobial against SMRSA and EMRSA (-1.2 % of the DMSO (D) control).
- isoquinoline alkaloids
- berberis brevissima
- berberis parkeriana
- fractions and compounds
Ali, S., Igoli, J., Clements, C., Semaan, D., Almazeb, M., Rashid, M-U., Shah, S. Q., Ferro, V., Gray, A., & Khan, M. R. (2013). Antidiabetic and antimicrobial activities of fractions and compounds isolated from Berberis brevissima Jafri and Berberis parkeriana Schneid. Bangladesh Journal of Pharmacology, 8(3), 336-342. https://doi.org/10.3329/bjp.v8i3.13888