Antibiotic resistance gene abundances correlate with metal and geochemical conditions in Scottish soils

Charles W. Knapp, Seánín M. McCluskey, Brajesh K. Singh, Colin D. Campbell, Gordon Hudson, David W. Graham

Research output: Contribution to journalArticle

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Abstract

The vast majority of antibiotic resistant genes (ARG) acquired by human pathogens have originated from the natural environment. Therefore, understanding factors that influence intrinsic levels of ARG in the environment could be epidemiologically significant. The selection for metal resistance often promotes AR in exposed organisms; however, the relationship between metal levels in nature and the intrinsic presence of ARG has not been fully assessed. Here, we quantified, using qPCR, the abundance of eleven ARG and compared their levels with geochemical conditions in randomly selected soils from a Scottish archive. Many ARG positively correlated with soil copper levels, with approximately half being highly significant (p<0.05); whereas chromium, nickel, lead, and iron also significantly correlated with specific ARG. Results show that geochemical metal conditions innately influence the potential for AR in soil. We suggest soil geochemical data might be used to estimate baseline gene presence on local, regional and global scales within epidemiological risk studies related to AR transmission from the environment.
Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalPLoS One
Volume6
Issue number11
DOIs
Publication statusPublished - 9 Nov 2011

Fingerprint

Microbial Drug Resistance
antibiotic resistance
Soil
Genes
Metals
metals
Anti-Bacterial Agents
Soils
antibiotics
soil
genes
Intrinsic Factor
metal tolerance
Chromium
Pathogens
Nickel
nickel
chromium
Copper
Epidemiologic Studies

Keywords

  • antibiotic resistance gene
  • metal
  • geochemical conditions
  • scottish soils

Cite this

Knapp, Charles W. ; McCluskey, Seánín M. ; Singh, Brajesh K. ; Campbell, Colin D. ; Hudson, Gordon ; Graham, David W. / Antibiotic resistance gene abundances correlate with metal and geochemical conditions in Scottish soils. In: PLoS One. 2011 ; Vol. 6, No. 11. pp. 1-6.
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Antibiotic resistance gene abundances correlate with metal and geochemical conditions in Scottish soils. / Knapp, Charles W.; McCluskey, Seánín M.; Singh, Brajesh K.; Campbell, Colin D.; Hudson, Gordon; Graham, David W.

In: PLoS One, Vol. 6, No. 11, 09.11.2011, p. 1-6.

Research output: Contribution to journalArticle

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