Another look at the mechanism involving trimeric dUTPases in Staphylococcus aureus pathogenicity island induction involves novel players in the party

Elisa Maiques, Nuria Quiles-Puchalt, Jorge Donderis, J. Rafael Ciges-Tomas, Christian Alite, Janine Z. Bowring, Suzanne Humphrey, José R. Penadés, Alberto Marina*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)
2 Downloads (Pure)

Abstract

We have recently proposed that the trimeric staphylococcal phage encoded dUTPases (Duts) are signaling molecules that act analogously to eukaryotic G-proteins, using dUTP as a second messenger. To perform this regulatory role, the Duts require their characteristic extra motif VI, present in all the staphylococcal phage coded trimeric Duts, as well as the strongly conserved Dut motif V. Recently, however, an alternative model involving Duts in the transfer of the staphylococcal islands (SaPIs) has been suggested, questioning the implication of motifs V and VI. Here, using state-of the-art techniques, we have revisited the proposed models. Our results confirm that the mechanism by which the Duts derepress the SaPI cycle depends on dUTP and involves both motifs V and VI, as we have previously proposed. Surprisingly, the conserved Dut motif IV is also implicated in SaPI derepression. However, and in agreement with the proposed alternative model, the dUTP inhibits rather than inducing the process, as we had initially proposed. In summary, our results clarify, validate and establish the mechanism by which the Duts perform regulatory functions.

Original languageEnglish
Pages (from-to)5457-5469
Number of pages13
JournalNucleic Acids Research
Volume44
Issue number11
Early online date25 Apr 2016
DOIs
Publication statusPublished - 20 Jun 2016

Keywords

  • staphylococcal phage
  • dUTPases
  • staphylococcal islands
  • signaling molecules

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