Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 78-83 |
| Number of pages | 5 |
| Journal | Journal of Food Composition and Analysis |
| Volume | 21 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 |
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Keywords
- sugars
- propolis
- bee pollen
- ligand exchange chromatography
- pulsed amperometric detection
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Analysis of sugars in bee pollen and propolis by ligand exchange chromatography in combination with pulsed amperometric detection and mass spectrometry. / Wei, L.Q.; Watson, D.G.; Fearnley, J.
In: Journal of Food Composition and Analysis, Vol. 21, No. 1, 2008, p. 78-83.Research output: Contribution to journal › Article
TY - JOUR
T1 - Analysis of sugars in bee pollen and propolis by ligand exchange chromatography in combination with pulsed amperometric detection and mass spectrometry
AU - Wei, L.Q.
AU - Watson, D.G.
AU - Fearnley, J.
PY - 2008
Y1 - 2008
N2 - The analysis of sugars in the natural products propolis and bee pollen was carried by high-performance thin layer chromatography (HPTLC), high-performance ligand exchange chromatography with pulsed amperometric detection (HPLEC-PAD) and HPLEC coupled to liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) in the positive ion mode. Eleven samples of propolis were analysed, most of which contained no sugars or only traces of sugars. Similar analysis was performed on five samples of bee pollen which were found to contain glucose, fructose and sucrose. These sugars were quantified by HPLEC-PAD. LC-ESI-MS analysis also indicated small amounts of a trisaccharide in the samples. Calibration curves were linear in the range 0.005-0.5 mg/ml and the LOD for all the sugars was <0.005 mg/ml. The positive ESI-MS of the sugars extracted from bee pollen yielded mainly Ca2+ adducts of sugars, which afforded good sensitivity for their detection.
AB - The analysis of sugars in the natural products propolis and bee pollen was carried by high-performance thin layer chromatography (HPTLC), high-performance ligand exchange chromatography with pulsed amperometric detection (HPLEC-PAD) and HPLEC coupled to liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) in the positive ion mode. Eleven samples of propolis were analysed, most of which contained no sugars or only traces of sugars. Similar analysis was performed on five samples of bee pollen which were found to contain glucose, fructose and sucrose. These sugars were quantified by HPLEC-PAD. LC-ESI-MS analysis also indicated small amounts of a trisaccharide in the samples. Calibration curves were linear in the range 0.005-0.5 mg/ml and the LOD for all the sugars was <0.005 mg/ml. The positive ESI-MS of the sugars extracted from bee pollen yielded mainly Ca2+ adducts of sugars, which afforded good sensitivity for their detection.
KW - sugars
KW - propolis
KW - bee pollen
KW - ligand exchange chromatography
KW - pulsed amperometric detection
UR - http://dx.doi.org/10.1016/j.jfca.2007.07.001
U2 - 10.1016/j.jfca.2007.07.001
DO - 10.1016/j.jfca.2007.07.001
M3 - Article
VL - 21
SP - 78
EP - 83
JO - Journal of Food Composition and Analysis
JF - Journal of Food Composition and Analysis
SN - 0889-1575
IS - 1
ER -