Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach

Annalisa De Angelis; Maria Antonietta Ferrara; Giuseppe Di Caprio; Stefano Manago; Luigi Sirleto; Giuseppe Coppola; Anna Chiara De Luca

doi:10.1109/AEIT.2015.7415259

Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach

Annalisa De Angelis, Maria Antonietta Ferrara, Giuseppe Di Caprio, Stefano Manago, Luigi Sirleto, Giuseppe Coppola, Anna Chiara De Luca

Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

1 Citation (Scopus)

Abstract

In this work, an approach based on digital holography (DH) combined with Raman spectroscopy (RS) is used for a complete label-free analysis of single bovine spermatozoa. DH allows to obtain high-resolution images of bovine sperm from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Quantitative 3D reconstructions of sperm head, both normal and anomalous, have been studied and an unexpected structure of the post-acrosomal region of the head has been detected. Raman imaging analysis have also confirmed such anomalies, suggesting the protein vibrations as associated Raman marker of the defect.

Original language	English
Title of host publication	2015 AEIT International Annual Conference, AEIT 2015
Place of Publication	Piscataway, NJ
Number of pages	6
ISBN (Electronic)	9788887237283
DOIs	https://doi.org/10.1109/AEIT.2015.7415259
Publication status	Published - 25 Feb 2016
Event	AEIT International Annual Conference: A Sustainable Development in the Mediterranean Area, AEIT 2015 - Naples, Italy Duration: 14 Oct 2015 → 16 Oct 2015

Conference

Conference	AEIT International Annual Conference: A Sustainable Development in the Mediterranean Area, AEIT 2015
Country/Territory	Italy
City	Naples
Period	14/10/15 → 16/10/15

Keywords

biophotonics
digital holography microscopy
Raman imaging
Raman spectroscopy
spermatozoa

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/AEIT.2015.7415259

Cite this

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title = "Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach",

abstract = "In this work, an approach based on digital holography (DH) combined with Raman spectroscopy (RS) is used for a complete label-free analysis of single bovine spermatozoa. DH allows to obtain high-resolution images of bovine sperm from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Quantitative 3D reconstructions of sperm head, both normal and anomalous, have been studied and an unexpected structure of the post-acrosomal region of the head has been detected. Raman imaging analysis have also confirmed such anomalies, suggesting the protein vibrations as associated Raman marker of the defect.",

keywords = "biophotonics, digital holography microscopy, Raman imaging, Raman spectroscopy, spermatozoa",

author = "{De Angelis}, Annalisa and Ferrara, {Maria Antonietta} and {Di Caprio}, Giuseppe and Stefano Manago and Luigi Sirleto and Giuseppe Coppola and {De Luca}, {Anna Chiara}",

year = "2016",

month = feb,

day = "25",

doi = "10.1109/AEIT.2015.7415259",

language = "English",

booktitle = "2015 AEIT International Annual Conference, AEIT 2015",

note = "AEIT International Annual Conference: A Sustainable Development in the Mediterranean Area, AEIT 2015 ; Conference date: 14-10-2015 Through 16-10-2015",

}

De Angelis, A, Ferrara, MA, Di Caprio, G, Manago, S, Sirleto, L, Coppola, G & De Luca, AC 2016, Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach. in 2015 AEIT International Annual Conference, AEIT 2015., 7415259, Piscataway, NJ, AEIT International Annual Conference: A Sustainable Development in the Mediterranean Area, AEIT 2015, Naples, Italy, 14/10/15. https://doi.org/10.1109/AEIT.2015.7415259

TY - GEN

T1 - Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach

AU - De Angelis, Annalisa

AU - Ferrara, Maria Antonietta

AU - Di Caprio, Giuseppe

AU - Manago, Stefano

AU - Sirleto, Luigi

AU - Coppola, Giuseppe

AU - De Luca, Anna Chiara

PY - 2016/2/25

Y1 - 2016/2/25

N2 - In this work, an approach based on digital holography (DH) combined with Raman spectroscopy (RS) is used for a complete label-free analysis of single bovine spermatozoa. DH allows to obtain high-resolution images of bovine sperm from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Quantitative 3D reconstructions of sperm head, both normal and anomalous, have been studied and an unexpected structure of the post-acrosomal region of the head has been detected. Raman imaging analysis have also confirmed such anomalies, suggesting the protein vibrations as associated Raman marker of the defect.

AB - In this work, an approach based on digital holography (DH) combined with Raman spectroscopy (RS) is used for a complete label-free analysis of single bovine spermatozoa. DH allows to obtain high-resolution images of bovine sperm from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Quantitative 3D reconstructions of sperm head, both normal and anomalous, have been studied and an unexpected structure of the post-acrosomal region of the head has been detected. Raman imaging analysis have also confirmed such anomalies, suggesting the protein vibrations as associated Raman marker of the defect.

KW - biophotonics

KW - digital holography microscopy

KW - Raman imaging

KW - Raman spectroscopy

KW - spermatozoa

U2 - 10.1109/AEIT.2015.7415259

DO - 10.1109/AEIT.2015.7415259

M3 - Conference contribution book

AN - SCOPUS:84964832748

BT - 2015 AEIT International Annual Conference, AEIT 2015

CY - Piscataway, NJ

T2 - AEIT International Annual Conference: A Sustainable Development in the Mediterranean Area, AEIT 2015

Y2 - 14 October 2015 through 16 October 2015

ER -

Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach

Abstract

Conference

Keywords

UN SDGs

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