An RNA splicing enhancer that does not act by looping

Helen  Lewis; Andrew Perrett; Glenn Burley; Ian  Eperon

doi:10.1002/anie.201202932

An RNA splicing enhancer that does not act by looping

Helen Lewis, Andrew Perrett, Glenn Burley, Ian Eperon

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Do the proteins bound to an enhancer site on pre-mRNA interact directly with the splice site by diffusion (looping), as is generally accepted, or does the intervening RNA play a role (see scheme)? By inserting a PEG linker between an enhancer sequence and alternative splice sites, the interaction of these two elements can be studied. Intervening RNA was essential for the enhancer activity, which rules out the looping model.

Original language	English
Pages (from-to)	9800–9803
Number of pages	4
Journal	Angewandte Chemie International Edition
Volume	51
Issue number	39
Early online date	31 Aug 2012
DOIs	https://doi.org/10.1002/anie.201202932
Publication status	Published - 24 Sept 2012

Keywords

click chemistry
pre-RNA splicing
mRNA
oligonucleotides
splicing enhancers
looping
rna splicing enhancer

Access to Document

10.1002/anie.201202932

Cite this

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AU - Eperon, Ian

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KW - click chemistry

KW - pre-RNA splicing

KW - mRNA

KW - oligonucleotides

KW - splicing enhancers

KW - looping

KW - rna splicing enhancer

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An RNA splicing enhancer that does not act by looping

Abstract

Keywords

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New Molecular Tools For The 21st Century: Molecular Design Of New DNA-Based Devices

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An RNA splicing enhancer that does not act by looping

Abstract

Keywords

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New Molecular Tools For The 21st Century: Molecular Design Of New DNA-Based Devices

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