An RNA splicing enhancer that does not act by looping

Helen Lewis, Andrew Perrett, Glenn Burley, Ian Eperon

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Do the proteins bound to an enhancer site on pre-mRNA interact directly with the splice site by diffusion (looping), as is generally accepted, or does the intervening RNA play a role (see scheme)? By inserting a PEG linker between an enhancer sequence and alternative splice sites, the interaction of these two elements can be studied. Intervening RNA was essential for the enhancer activity, which rules out the looping model.
Original languageEnglish
Pages (from-to)9800–9803
Number of pages4
JournalAngewandte Chemie International Edition
Volume51
Issue number39
Early online date31 Aug 2012
DOIs
Publication statusPublished - 24 Sep 2012

Keywords

  • click chemistry
  • pre-RNA splicing
  • mRNA
  • oligonucleotides
  • splicing enhancers
  • looping
  • rna splicing enhancer

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