An RNA Aptamer that Induces Transcription

A. Hunsicker, M. Steber, G. Mayer, J. Meitert, M. Klotzsche, M. Blind, W. Hillen, C. Berens

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We identified an RNA aptamer that induces TetR-controlled gene expression in Escherichia coli when expressed in the cell. The aptamer was found by a combined approach of in vitro selection for TetR binding and in vivo screening for TetR induction. The smallest active aptamer folds into a stem-loop with an internal loop interrupting the stem. Mutational analysis in vivo and in-line probing in vitro reveal this loop to be the protein binding site. The TetR-inducing activity of the aptamer directly correlates with its stability and the best construct is as efficient as the natural inducer tetracycline. Because of its small size, high induction efficiency, and the stability of the TetR aptamer under in vivo conditions, it is well suited to be an alternative RNA-based inducer of TetR-controlled gene expression.
LanguageEnglish
Pages173-180
Number of pages7
JournalChemistry and Biology
Volume16
DOIs
Publication statusPublished - 2009

Fingerprint

Nucleotide Aptamers
Transcription
Gene expression
Gene Expression
Tetracycline
Protein Binding
Escherichia coli
Screening
Binding Sites
RNA
In Vitro Techniques

Keywords

  • TN10 tet repressor
  • in-vitro
  • selection
  • binding
  • recognition
  • operator
  • ligands
  • system
  • motif
  • vivo

Cite this

Hunsicker, A., Steber, M., Mayer, G., Meitert, J., Klotzsche, M., Blind, M., ... Berens, C. (2009). An RNA Aptamer that Induces Transcription. Chemistry and Biology, 16, 173-180. https://doi.org/10.1016/j.chembiol.2008.12.008
Hunsicker, A. ; Steber, M. ; Mayer, G. ; Meitert, J. ; Klotzsche, M. ; Blind, M. ; Hillen, W. ; Berens, C. / An RNA Aptamer that Induces Transcription. In: Chemistry and Biology. 2009 ; Vol. 16. pp. 173-180.
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Hunsicker, A, Steber, M, Mayer, G, Meitert, J, Klotzsche, M, Blind, M, Hillen, W & Berens, C 2009, 'An RNA Aptamer that Induces Transcription' Chemistry and Biology, vol. 16, pp. 173-180. https://doi.org/10.1016/j.chembiol.2008.12.008

An RNA Aptamer that Induces Transcription. / Hunsicker, A.; Steber, M.; Mayer, G.; Meitert, J.; Klotzsche, M.; Blind, M.; Hillen, W.; Berens, C.

In: Chemistry and Biology, Vol. 16, 2009, p. 173-180.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An RNA Aptamer that Induces Transcription

AU - Hunsicker, A.

AU - Steber, M.

AU - Mayer, G.

AU - Meitert, J.

AU - Klotzsche, M.

AU - Blind, M.

AU - Hillen, W.

AU - Berens, C.

N1 - Strathprints' policy is to record up to 8 authors per publication, plus any additional authors based at the University of Strathclyde. More authors may be listed on the official publication than appear in the Strathprints' record.

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AB - We identified an RNA aptamer that induces TetR-controlled gene expression in Escherichia coli when expressed in the cell. The aptamer was found by a combined approach of in vitro selection for TetR binding and in vivo screening for TetR induction. The smallest active aptamer folds into a stem-loop with an internal loop interrupting the stem. Mutational analysis in vivo and in-line probing in vitro reveal this loop to be the protein binding site. The TetR-inducing activity of the aptamer directly correlates with its stability and the best construct is as efficient as the natural inducer tetracycline. Because of its small size, high induction efficiency, and the stability of the TetR aptamer under in vivo conditions, it is well suited to be an alternative RNA-based inducer of TetR-controlled gene expression.

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KW - binding

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Hunsicker A, Steber M, Mayer G, Meitert J, Klotzsche M, Blind M et al. An RNA Aptamer that Induces Transcription. Chemistry and Biology. 2009;16:173-180. https://doi.org/10.1016/j.chembiol.2008.12.008