Projects per year
Abstract
Organic semiconductor lasers are a sensitive biosensing platform that responds to specific biomolecule binding event. So far, such biosensors have utilized protein-based interactions for surface functionalization but a nucleic acid-based strategy would considerably widen their utility as a general biodiagnostic platform. This manuscript reports two important advances for DNA-based sensing using an organic semiconductor (OS) distributed feedback (DFB) laser. Firstly, the immobilization of alkyne-tagged 12/18-mer oligodeoxyribonucleotide (ODN) probes by Cu-catalyzed azide alkyne cycloaddition (CuAAC) or ‘click chemistry’ onto an 80-nm thick OS laser film modified with an azide-presenting polyelectrolyte monolayer is presented. Secondly, sequence-selective binding to these immobilized probes with complementary ODN functionalized silver nanoparticles, is detected. As binding occurs, the nanoparticles increase the optical losses of the laser mode through plasmonic scattering and absorption, and this causes a rise in the threshold pump energy required for laser action that is proportional to the analyte concentration. By monitoring this threshold, detection of the complementary ODN target down to 11.5 pM is achieved. This complementary binding on the laser surface is independently confirmed through surface-enhanced Raman spectroscopy (SERS).
Original language | English |
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Number of pages | 8 |
Journal | Langmuir |
Early online date | 18 Sep 2018 |
DOIs | |
Publication status | E-pub ahead of print - 18 Sep 2018 |
Keywords
- organic semiconductor lasers
- DNA-based sensing
- surface-enhanced Raman spectroscopy (SERS)
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Projects
- 2 Finished
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Single-molecule studies of light-emitting polymers: observing and manipulating polymer conformation in solution
Skabara, P.
EPSRC (Engineering and Physical Sciences Research Council)
1/02/16 → 31/01/19
Project: Research
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Medical Devices Doctoral Training Centre Renewal | Smith, Nicholas
Riches, P., Stankovic, V. & Smith, N.
EPSRC (Engineering and Physical Sciences Research Council)
1/10/11 → 14/09/16
Project: Research Studentship - Internally Allocated
Datasets
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AgNP DFB laser assesment
McConnell, G. (Creator), Laurand, N. (Supervisor) & Burley, G. (Supervisor), University of Strathclyde, 19 Sep 2018
DOI: 10.15129/1316da75-360d-4cf7-b9e3-57d0b18d2190
Dataset