An organic semiconductor laser platform for the detection of DNA by AgNP plasmonic enhancement

G. McConnell, S. Mabbott, A. L. Kanibolotskyy, P. J. Skabara, D. Graham, G. A. Burley, N. Laurand

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Organic semiconductor lasers are a sensitive biosensing platform that responds to specific biomolecule binding event. So far, such biosensors have utilized protein-based interactions for surface functionalization but a nucleic acid-based strategy would considerably widen their utility as a general biodiagnostic platform. This manuscript reports two important advances for DNA-based sensing using an organic semiconductor (OS) distributed feedback (DFB) laser. Firstly, the immobilization of alkyne-tagged 12/18-mer oligodeoxyribonucleotide (ODN) probes by Cu-catalyzed azide alkyne cycloaddition (CuAAC) or ‘click chemistry’ onto an 80-nm thick OS laser film modified with an azide-presenting polyelectrolyte monolayer is presented. Secondly, sequence-selective binding to these immobilized probes with complementary ODN functionalized silver nanoparticles, is detected. As binding occurs, the nanoparticles increase the optical losses of the laser mode through plasmonic scattering and absorption, and this causes a rise in the threshold pump energy required for laser action that is proportional to the analyte concentration. By monitoring this threshold, detection of the complementary ODN target down to 11.5 pM is achieved. This complementary binding on the laser surface is independently confirmed through surface-enhanced Raman spectroscopy (SERS).
LanguageEnglish
Number of pages8
JournalLangmuir
Early online date18 Sep 2018
DOIs
Publication statusE-pub ahead of print - 18 Sep 2018

Fingerprint

organic lasers
Semiconducting organic compounds
organic semiconductors
Semiconductor lasers
DNA
Alkynes
Azides
deoxyribonucleic acid
platforms
Oligodeoxyribonucleotides
semiconductor lasers
augmentation
alkynes
Nanoparticles
Optical losses
Cycloaddition
Oligonucleotide Probes
Lasers
Distributed feedback lasers
Nucleic acids

Keywords

  • organic semiconductor lasers
  • DNA-based sensing
  • surface-enhanced Raman spectroscopy (SERS)

Cite this

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title = "An organic semiconductor laser platform for the detection of DNA by AgNP plasmonic enhancement",
abstract = "Organic semiconductor lasers are a sensitive biosensing platform that responds to specific biomolecule binding event. So far, such biosensors have utilized protein-based interactions for surface functionalization but a nucleic acid-based strategy would considerably widen their utility as a general biodiagnostic platform. This manuscript reports two important advances for DNA-based sensing using an organic semiconductor (OS) distributed feedback (DFB) laser. Firstly, the immobilization of alkyne-tagged 12/18-mer oligodeoxyribonucleotide (ODN) probes by Cu-catalyzed azide alkyne cycloaddition (CuAAC) or ‘click chemistry’ onto an 80-nm thick OS laser film modified with an azide-presenting polyelectrolyte monolayer is presented. Secondly, sequence-selective binding to these immobilized probes with complementary ODN functionalized silver nanoparticles, is detected. As binding occurs, the nanoparticles increase the optical losses of the laser mode through plasmonic scattering and absorption, and this causes a rise in the threshold pump energy required for laser action that is proportional to the analyte concentration. By monitoring this threshold, detection of the complementary ODN target down to 11.5 pM is achieved. This complementary binding on the laser surface is independently confirmed through surface-enhanced Raman spectroscopy (SERS).",
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An organic semiconductor laser platform for the detection of DNA by AgNP plasmonic enhancement. / McConnell, G.; Mabbott, S.; Kanibolotskyy, A. L.; Skabara, P. J.; Graham, D.; Burley, G. A.; Laurand, N.

In: Langmuir, 18.09.2018.

Research output: Contribution to journalArticle

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