An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy

M. J. Baker, N. W. Clarke, D. Démoulin, J.M. Nicholson, F.M. Lyng, Hugh J. Byrne, Claire A. Hart, M.D. Brown, N.W. Clarke, P. Gardner

Research output: Contribution to journalArticle

29 Citations (Scopus)
43 Downloads (Pure)

Abstract

Interest in developing robust, quicker and easier diagnostic tests for cancer has lead to an increased use of Fourier transform infrared (FTIR) spectroscopy to meet that need. In this study we present the use of different experimental modes of infrared spectroscopy to investigate the RWPE human prostate epithelial cell line family which are derived from the same source but differ in their mode of transformation and their mode of invasive phenotype. Importantly, analysis of the infrared spectra obtained using different experimental modes of infrared spectroscopy produces similar results. The RWPE family of cell lines can be separated into groups based upon the method of cell transformation rather than the resulting invasiveness/aggressiveness of the cell line. The study also demonstrates the possibility of using a genetic algorithm as a possible standardised pre-processing step and raises the important question of the usefulness of cell lines to create a biochemical model of prostate cancer progression.

Original languageEnglish
Pages (from-to)887-894
Number of pages8
JournalAnalyst
Volume135
Issue number5
Early online date4 Mar 2010
DOIs
Publication statusPublished - 3 May 2010

Fingerprint

Fourier Transform Infrared Spectroscopy
FTIR spectroscopy
infrared spectroscopy
Fourier transform infrared spectroscopy
Prostate
cancer
Cells
Cell Line
Spectrum Analysis
invasiveness
Infrared spectroscopy
aggression
genetic algorithm
phenotype
Genetic algorithms
Routine Diagnostic Tests
Infrared radiation
Prostatic Neoplasms
Epithelial Cells
Processing

Keywords

  • cancer diagnostic tests
  • cell lines
  • infrared spectroscopy

Cite this

Baker, M. J., Clarke, N. W., Démoulin, D., Nicholson, J. M., Lyng, F. M., Byrne, H. J., ... Gardner, P. (2010). An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy. Analyst, 135(5), 887-894. https://doi.org/10.1039/b920385k
Baker, M. J. ; Clarke, N. W. ; Démoulin, D. ; Nicholson, J.M. ; Lyng, F.M. ; Byrne, Hugh J. ; Hart, Claire A. ; Brown, M.D. ; Clarke, N.W. ; Gardner, P. / An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy. In: Analyst. 2010 ; Vol. 135, No. 5. pp. 887-894.
@article{b42acd4f62e54fd4bba89877ac820b88,
title = "An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy",
abstract = "Interest in developing robust, quicker and easier diagnostic tests for cancer has lead to an increased use of Fourier transform infrared (FTIR) spectroscopy to meet that need. In this study we present the use of different experimental modes of infrared spectroscopy to investigate the RWPE human prostate epithelial cell line family which are derived from the same source but differ in their mode of transformation and their mode of invasive phenotype. Importantly, analysis of the infrared spectra obtained using different experimental modes of infrared spectroscopy produces similar results. The RWPE family of cell lines can be separated into groups based upon the method of cell transformation rather than the resulting invasiveness/aggressiveness of the cell line. The study also demonstrates the possibility of using a genetic algorithm as a possible standardised pre-processing step and raises the important question of the usefulness of cell lines to create a biochemical model of prostate cancer progression.",
keywords = "cancer diagnostic tests, cell lines, infrared spectroscopy",
author = "Baker, {M. J.} and Clarke, {N. W.} and D. D{\'e}moulin and J.M. Nicholson and F.M. Lyng and Byrne, {Hugh J.} and Hart, {Claire A.} and M.D. Brown and N.W. Clarke and P. Gardner",
year = "2010",
month = "5",
day = "3",
doi = "10.1039/b920385k",
language = "English",
volume = "135",
pages = "887--894",
journal = "Analyst",
issn = "0003-2654",
number = "5",

}

Baker, MJ, Clarke, NW, Démoulin, D, Nicholson, JM, Lyng, FM, Byrne, HJ, Hart, CA, Brown, MD, Clarke, NW & Gardner, P 2010, 'An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy', Analyst, vol. 135, no. 5, pp. 887-894. https://doi.org/10.1039/b920385k

An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy. / Baker, M. J.; Clarke, N. W.; Démoulin, D.; Nicholson, J.M.; Lyng, F.M.; Byrne, Hugh J.; Hart, Claire A.; Brown, M.D.; Clarke, N.W.; Gardner, P.

In: Analyst, Vol. 135, No. 5, 03.05.2010, p. 887-894.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy

AU - Baker, M. J.

AU - Clarke, N. W.

AU - Démoulin, D.

AU - Nicholson, J.M.

AU - Lyng, F.M.

AU - Byrne, Hugh J.

AU - Hart, Claire A.

AU - Brown, M.D.

AU - Clarke, N.W.

AU - Gardner, P.

PY - 2010/5/3

Y1 - 2010/5/3

N2 - Interest in developing robust, quicker and easier diagnostic tests for cancer has lead to an increased use of Fourier transform infrared (FTIR) spectroscopy to meet that need. In this study we present the use of different experimental modes of infrared spectroscopy to investigate the RWPE human prostate epithelial cell line family which are derived from the same source but differ in their mode of transformation and their mode of invasive phenotype. Importantly, analysis of the infrared spectra obtained using different experimental modes of infrared spectroscopy produces similar results. The RWPE family of cell lines can be separated into groups based upon the method of cell transformation rather than the resulting invasiveness/aggressiveness of the cell line. The study also demonstrates the possibility of using a genetic algorithm as a possible standardised pre-processing step and raises the important question of the usefulness of cell lines to create a biochemical model of prostate cancer progression.

AB - Interest in developing robust, quicker and easier diagnostic tests for cancer has lead to an increased use of Fourier transform infrared (FTIR) spectroscopy to meet that need. In this study we present the use of different experimental modes of infrared spectroscopy to investigate the RWPE human prostate epithelial cell line family which are derived from the same source but differ in their mode of transformation and their mode of invasive phenotype. Importantly, analysis of the infrared spectra obtained using different experimental modes of infrared spectroscopy produces similar results. The RWPE family of cell lines can be separated into groups based upon the method of cell transformation rather than the resulting invasiveness/aggressiveness of the cell line. The study also demonstrates the possibility of using a genetic algorithm as a possible standardised pre-processing step and raises the important question of the usefulness of cell lines to create a biochemical model of prostate cancer progression.

KW - cancer diagnostic tests

KW - cell lines

KW - infrared spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=77951545365&partnerID=8YFLogxK

U2 - 10.1039/b920385k

DO - 10.1039/b920385k

M3 - Article

VL - 135

SP - 887

EP - 894

JO - Analyst

JF - Analyst

SN - 0003-2654

IS - 5

ER -

Baker MJ, Clarke NW, Démoulin D, Nicholson JM, Lyng FM, Byrne HJ et al. An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy. Analyst. 2010 May 3;135(5):887-894. https://doi.org/10.1039/b920385k