An investigation into the simultaneous enzymatic and SERRS properties of silver nanoparticles

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Investigation into the use of artificial enzymes has become an increasingly popular area of research due to the numerous advantages offered in comparison to protein enzymes. One particular area of research interest involves the use of metal nanoparticles as artificial enzymes. The peroxidase-like activity of a variety of nanoparticles has recently been shown and their use in a range of assay formats for the detection of various analytes has been explored. Herein the enzyme mimicking activity of silver nanoparticles is investigated using the peroxidase substrate 3,3′,5′,5′-tetramethylbenzidine (TMB). The peroxidase-like nature of these nanoparticles can be used in combination with surface enhanced resonance Raman scattering (SERRS) to provide a novel spectroscopic method of analysis. Negatively charged silver nanoparticles were investigated in combination with TMB using SERRS and it was found that upon formation of the oxidation intermediate of TMB, small clusters of positively charged nanoparticles were formed. The enzyme like behaviour of silver nanoparticles along with their use as a SERRS substrate is combined to demonstrate a simple and rapid method for the direct detection of hydrogen peroxide with a detection limit of 100 nM.
LanguageEnglish
Pages6347-6353
Number of pages7
JournalAnalyst
Volume138
Issue number21
DOIs
Publication statusPublished - 7 Nov 2013

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Raman Spectrum Analysis
Silver
Nanoparticles
Raman scattering
silver
scattering
Enzymes
Peroxidase
enzyme
Metal Nanoparticles
Metal nanoparticles
substrate
Enzyme activity
Substrates
Research
Hydrogen peroxide
Hydrogen Peroxide
Limit of Detection
nanoparticle
Assays

Keywords

  • artificial enzymes
  • protein enzymes
  • surface enhanced resonance raman scattering

Cite this

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An investigation into the simultaneous enzymatic and SERRS properties of silver nanoparticles. / McKeating, K.S.; Sloan-Dennison, S.; Graham, D.; Faulds, K.

In: Analyst, Vol. 138, No. 21, 07.11.2013, p. 6347-6353.

Research output: Contribution to journalArticle

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