An insulin mediator preparation serves to stimulate the cyclic GMP activated cyclic AMP phosphodiesterase rather than other purified insulin-activated cyclic AMP phosphodiesterases

N J Pyne, M D Houslay

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6 Citations (Scopus)

Abstract

An insulin mediator preparation was obtained from rat hepatocytes which had been treated with insulin. This preparation inhibited adenylate cyclase activity. It stimulated the activity of homogeneous preparations of both the cytosolic and membrane-bound forms of rat liver cyclic GMP-activated cyclic AMP phosphodiesterase. It failed to activate homogeneous preparations of both the peripheral plasma membrane and 'dense-vesicle' cyclic AMP phosphodiesterases. The insulin mediator preparation stimulated cyclic GMP-activated cyclic AMP phosphodiesterase activity in a dose-dependent fashion with a hill coefficient of 0.46. Insulin caused the dose-dependent production of mediator activity in intact hepatocytes with a Ka of 9 pM, although concentrations of insulin greater than 10 nM progressively reduced stimulatory activity.
LanguageEnglish
Pages290-296
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume156
Issue number1
DOIs
Publication statusPublished - 14 Oct 1988

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Cyclic GMP
Phosphoric Diester Hydrolases
Cyclic AMP
Insulin
Rats
Hepatocytes
Cell membranes
Adenylyl Cyclases
Liver
Cell Membrane
Membranes

Keywords

  • 3',5'-cyclic-AMP phosphodiesterases
  • adenylate cyclase
  • animals
  • cells, cultured
  • cyclic GMP
  • glucagon
  • insulin
  • isoenzymes
  • kinetics
  • liver
  • second messenger systems

Cite this

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title = "An insulin mediator preparation serves to stimulate the cyclic GMP activated cyclic AMP phosphodiesterase rather than other purified insulin-activated cyclic AMP phosphodiesterases",
abstract = "An insulin mediator preparation was obtained from rat hepatocytes which had been treated with insulin. This preparation inhibited adenylate cyclase activity. It stimulated the activity of homogeneous preparations of both the cytosolic and membrane-bound forms of rat liver cyclic GMP-activated cyclic AMP phosphodiesterase. It failed to activate homogeneous preparations of both the peripheral plasma membrane and 'dense-vesicle' cyclic AMP phosphodiesterases. The insulin mediator preparation stimulated cyclic GMP-activated cyclic AMP phosphodiesterase activity in a dose-dependent fashion with a hill coefficient of 0.46. Insulin caused the dose-dependent production of mediator activity in intact hepatocytes with a Ka of 9 pM, although concentrations of insulin greater than 10 nM progressively reduced stimulatory activity.",
keywords = "3',5'-cyclic-AMP phosphodiesterases, adenylate cyclase, animals, cells, cultured, cyclic GMP, glucagon, insulin, isoenzymes, kinetics, liver, second messenger systems",
author = "Pyne, {N J} and Houslay, {M D}",
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T1 - An insulin mediator preparation serves to stimulate the cyclic GMP activated cyclic AMP phosphodiesterase rather than other purified insulin-activated cyclic AMP phosphodiesterases

AU - Pyne, N J

AU - Houslay, M D

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Y1 - 1988/10/14

N2 - An insulin mediator preparation was obtained from rat hepatocytes which had been treated with insulin. This preparation inhibited adenylate cyclase activity. It stimulated the activity of homogeneous preparations of both the cytosolic and membrane-bound forms of rat liver cyclic GMP-activated cyclic AMP phosphodiesterase. It failed to activate homogeneous preparations of both the peripheral plasma membrane and 'dense-vesicle' cyclic AMP phosphodiesterases. The insulin mediator preparation stimulated cyclic GMP-activated cyclic AMP phosphodiesterase activity in a dose-dependent fashion with a hill coefficient of 0.46. Insulin caused the dose-dependent production of mediator activity in intact hepatocytes with a Ka of 9 pM, although concentrations of insulin greater than 10 nM progressively reduced stimulatory activity.

AB - An insulin mediator preparation was obtained from rat hepatocytes which had been treated with insulin. This preparation inhibited adenylate cyclase activity. It stimulated the activity of homogeneous preparations of both the cytosolic and membrane-bound forms of rat liver cyclic GMP-activated cyclic AMP phosphodiesterase. It failed to activate homogeneous preparations of both the peripheral plasma membrane and 'dense-vesicle' cyclic AMP phosphodiesterases. The insulin mediator preparation stimulated cyclic GMP-activated cyclic AMP phosphodiesterase activity in a dose-dependent fashion with a hill coefficient of 0.46. Insulin caused the dose-dependent production of mediator activity in intact hepatocytes with a Ka of 9 pM, although concentrations of insulin greater than 10 nM progressively reduced stimulatory activity.

KW - 3',5'-cyclic-AMP phosphodiesterases

KW - adenylate cyclase

KW - animals

KW - cells, cultured

KW - cyclic GMP

KW - glucagon

KW - insulin

KW - isoenzymes

KW - kinetics

KW - liver

KW - second messenger systems

U2 - 10.1016/S0006-291X(88)80838-6

DO - 10.1016/S0006-291X(88)80838-6

M3 - Article

VL - 156

SP - 290

EP - 296

JO - Biochemical and Biophysical Research Communications

T2 - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 1090-2104

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