An experimental-theoretical analysis of protein adsorption on peptidomimetic polymer brushes

K. H. A. Lau, C. L. Ren, S. H. Park, I. Szleifer, P. B. Messersmith

Research output: Contribution to journalArticle

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Abstract

Surface-grafted water-soluble polymer brushes are being intensely investigated for preventing protein adsorption to improve biomedical device function, prevent marine fouling, and enable applications in biosensing and tissue engineering. In this contribution, we present an experimental-theoretical analysis of a peptidomimetic polymer brush system with regard to the critical brush density required for preventing protein adsorption at varying chain lengths. A mussel adhesive-inspired DOPA-Lys (DOPA = 3,4-dihydroxy-phenylalanine; Lys = lysine) pentapeptide surface grafting motif enabled aqueous deposition of our peptidomimetic polypeptoid brushes over a wide range of chain densities. Critical densities of 0.88 nm(-2) for a relatively short polypeptoid 10-mer to 0.42 nm(-2) for a SO-mer were identified from measurements of protein adsorption. The experiments were also compared with the protein adsorption isotherms predicted by a molecular theory. Excellent agreements in terms of both the polymer brush structure and the critical chain density were obtained. Furthermore, atomic force microscopy (AFM) imaging is shown to be useful in verifying the critical brush density for preventing protein adsorption. The present coanalysis of experimental and theoretical results demonstrates the significance of characterizing the critical brush density in evaluating the performance of an antifouling polymer brush system. The high fidelity of the agreement between the experiments and molecular theory also indicate that the theoretical approach presented can aid in the practical design of antifouling polymer brush systems.
Original languageEnglish
Pages (from-to)2288-2298
Number of pages11
JournalLangmuir
Volume28
Issue number4
Early online date22 Nov 2011
DOIs
Publication statusPublished - 2012

Fingerprint

Peptidomimetics
brushes
Brushes
Polymers
proteins
Proteins
Adsorption
adsorption
polymers
antifouling
molecular theory
phenylalanine
fouling
tissue engineering
lysine
Fouling
surface water
Phenylalanine
Surface waters
Chain length

Keywords

  • layers
  • transfer
  • radical
  • polymerization
  • self-assembled
  • monolayers
  • grafted
  • chain-length
  • peptoid
  • blood-plasma
  • surfaces
  • density
  • adhesion
  • polymers
  • oligomers

Cite this

Lau, K. H. A. ; Ren, C. L. ; Park, S. H. ; Szleifer, I. ; Messersmith, P. B. / An experimental-theoretical analysis of protein adsorption on peptidomimetic polymer brushes. In: Langmuir. 2012 ; Vol. 28, No. 4. pp. 2288-2298.
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author = "Lau, {K. H. A.} and Ren, {C. L.} and Park, {S. H.} and I. Szleifer and Messersmith, {P. B.}",
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Lau, KHA, Ren, CL, Park, SH, Szleifer, I & Messersmith, PB 2012, 'An experimental-theoretical analysis of protein adsorption on peptidomimetic polymer brushes', Langmuir, vol. 28, no. 4, pp. 2288-2298. https://doi.org/10.1021/la203905g

An experimental-theoretical analysis of protein adsorption on peptidomimetic polymer brushes. / Lau, K. H. A.; Ren, C. L.; Park, S. H.; Szleifer, I.; Messersmith, P. B.

In: Langmuir, Vol. 28, No. 4, 2012, p. 2288-2298.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An experimental-theoretical analysis of protein adsorption on peptidomimetic polymer brushes

AU - Lau, K. H. A.

AU - Ren, C. L.

AU - Park, S. H.

AU - Szleifer, I.

AU - Messersmith, P. B.

PY - 2012

Y1 - 2012

N2 - Surface-grafted water-soluble polymer brushes are being intensely investigated for preventing protein adsorption to improve biomedical device function, prevent marine fouling, and enable applications in biosensing and tissue engineering. In this contribution, we present an experimental-theoretical analysis of a peptidomimetic polymer brush system with regard to the critical brush density required for preventing protein adsorption at varying chain lengths. A mussel adhesive-inspired DOPA-Lys (DOPA = 3,4-dihydroxy-phenylalanine; Lys = lysine) pentapeptide surface grafting motif enabled aqueous deposition of our peptidomimetic polypeptoid brushes over a wide range of chain densities. Critical densities of 0.88 nm(-2) for a relatively short polypeptoid 10-mer to 0.42 nm(-2) for a SO-mer were identified from measurements of protein adsorption. The experiments were also compared with the protein adsorption isotherms predicted by a molecular theory. Excellent agreements in terms of both the polymer brush structure and the critical chain density were obtained. Furthermore, atomic force microscopy (AFM) imaging is shown to be useful in verifying the critical brush density for preventing protein adsorption. The present coanalysis of experimental and theoretical results demonstrates the significance of characterizing the critical brush density in evaluating the performance of an antifouling polymer brush system. The high fidelity of the agreement between the experiments and molecular theory also indicate that the theoretical approach presented can aid in the practical design of antifouling polymer brush systems.

AB - Surface-grafted water-soluble polymer brushes are being intensely investigated for preventing protein adsorption to improve biomedical device function, prevent marine fouling, and enable applications in biosensing and tissue engineering. In this contribution, we present an experimental-theoretical analysis of a peptidomimetic polymer brush system with regard to the critical brush density required for preventing protein adsorption at varying chain lengths. A mussel adhesive-inspired DOPA-Lys (DOPA = 3,4-dihydroxy-phenylalanine; Lys = lysine) pentapeptide surface grafting motif enabled aqueous deposition of our peptidomimetic polypeptoid brushes over a wide range of chain densities. Critical densities of 0.88 nm(-2) for a relatively short polypeptoid 10-mer to 0.42 nm(-2) for a SO-mer were identified from measurements of protein adsorption. The experiments were also compared with the protein adsorption isotherms predicted by a molecular theory. Excellent agreements in terms of both the polymer brush structure and the critical chain density were obtained. Furthermore, atomic force microscopy (AFM) imaging is shown to be useful in verifying the critical brush density for preventing protein adsorption. The present coanalysis of experimental and theoretical results demonstrates the significance of characterizing the critical brush density in evaluating the performance of an antifouling polymer brush system. The high fidelity of the agreement between the experiments and molecular theory also indicate that the theoretical approach presented can aid in the practical design of antifouling polymer brush systems.

KW - layers

KW - transfer

KW - radical

KW - polymerization

KW - self-assembled

KW - monolayers

KW - grafted

KW - chain-length

KW - peptoid

KW - blood-plasma

KW - surfaces

KW - density

KW - adhesion

KW - polymers

KW - oligomers

UR - http://pubs.acs.org/journal/langd5

U2 - 10.1021/la203905g

DO - 10.1021/la203905g

M3 - Article

VL - 28

SP - 2288

EP - 2298

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 4

ER -