An evaluation of six presumptive tests for blood, their specificity, sensitivity and effect on high molecular weight DNA

S.S. Tobe, N.D. Watson, N. Nic Daeid

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Luminol, leuchomalachite green, phenolphthalein, Hemastix®, Hemident™, and Bluestar© are all used as presumptive tests for blood. In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. Samples were tested for DNA to determine whether the presumptive tests damaged or destroyed DNA. The DNA loci tested were D2S1338 and D19S433. Leuchomalachite green had a sensitivity of 1:10,000, while the remaining tests were able to detect blood to a dilution of 1:100,000. Substances tested include saliva, semen, potato, tomato, tomato sauce, tomato sauce with meat, red onion, red kidney bean, horseradish, 0.1 M ascorbic acid, 5% bleach, 10% cupric sulfate, 10% ferric sulfate, and 10% nickel chloride. Of all the substances tested, not one of the household items reacted with every test; however, the chemicals did. DNA was recovered and amplified from luminol, phenolphthalein, Hemastix®, and Bluestar©, but not from leuchomalachite green or Hemident™.
LanguageEnglish
Pages102-109
Number of pages8
JournalJournal of Forensic Sciences
Volume52
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Hematologic Tests
Lycopersicon esculentum
Molecular Weight
Phenolphthalein
Sensitivity and Specificity
Luminol
DNA
Armoracia
Copper Sulfate
Onions
Phaseolus
Solanum tuberosum
Semen
Saliva
Ascorbic Acid

Keywords

  • forensic science
  • luminol
  • leuchomalachite green
  • phenolphthalein
  • Hemastix®
  • Hemident™
  • Bluestar©

Cite this

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title = "An evaluation of six presumptive tests for blood, their specificity, sensitivity and effect on high molecular weight DNA",
abstract = "Luminol, leuchomalachite green, phenolphthalein, Hemastix{\circledR}, Hemident™, and Bluestar{\circledC} are all used as presumptive tests for blood. In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. Samples were tested for DNA to determine whether the presumptive tests damaged or destroyed DNA. The DNA loci tested were D2S1338 and D19S433. Leuchomalachite green had a sensitivity of 1:10,000, while the remaining tests were able to detect blood to a dilution of 1:100,000. Substances tested include saliva, semen, potato, tomato, tomato sauce, tomato sauce with meat, red onion, red kidney bean, horseradish, 0.1 M ascorbic acid, 5{\%} bleach, 10{\%} cupric sulfate, 10{\%} ferric sulfate, and 10{\%} nickel chloride. Of all the substances tested, not one of the household items reacted with every test; however, the chemicals did. DNA was recovered and amplified from luminol, phenolphthalein, Hemastix{\circledR}, and Bluestar{\circledC}, but not from leuchomalachite green or Hemident™.",
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An evaluation of six presumptive tests for blood, their specificity, sensitivity and effect on high molecular weight DNA. / Tobe, S.S.; Watson, N.D.; Nic Daeid, N.

In: Journal of Forensic Sciences, Vol. 52, No. 1, 01.2007, p. 102-109.

Research output: Contribution to journalArticle

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