HPV genotyping is an important tool in the epidemiology and surveillance of HPV associated cancers and for the risk-stratification of HPV infections. HPV sign Genotyping Test (QIAGEN) is a new pyrosequencing assay for the detection and genotyping of HPV. The sensitivity and comparative performance of HPV sign was determined using a sample panel derived from histologically confirmed cervical lesions (cervical intraepithelial neoplasia 2 or worse) and oropharyngeal squamous cell carcinomas. Comparative analysis showed that 80% of cervical intraepithelial neoplasia 2+ and 81% of oropharyngeal squamous cell carcinomas were HPV positive by HPV sign compared to 100% of the cervical intraepithelial neoplasia 2+ and 81% of oropharyngeal squamous cell carcinomas by the digene HPV Genotyping RH Test (RH), and INNO-LiPA HPV Genotyping Extra assay respectively. Fewer genotypes were detected overall by HPV sign than via the relevant comparator assays (10 v 21 for cervical intraepithelial neoplasia 2+; 4 v 9 for oropharyngeal squamous cell carcinomas) and also fewer multiple infections (9 v 28 for cervical intraepithelial neoplasia 2+; 0 v 4 for oropharyngeal squamous cell carcinomas). HPV sign results were more compatible with the comparator assay for the oropharyngeal squamous cell carcinoma samples (100%) than for the cervical samples (73%). These results suggest that HPV sign in its current form is suited to samples that harbour multiple infection.
- oropharyngeal squamous cell carcinoma
Ward, C., Pedraza, J., Kavanagh, K., Johannessen, I., & Cuschieri, K. (2014). An evaluation of Qiagen HPV sign for the detection and genotyping of cervical lesions and oropharyngeal squamous cell carcinomas. Journal of Virological Methods, 207, 128-132. https://doi.org/10.1016/j.jviromet.2014.07.001