An empirical analysis of enzyme function reporting for experimental reproducibility: missing/incomplete information in published papers

Peter Halling, Paul F. Fitzpatrick, Frank M. Raushel, Johann Rohwer, Santiago Schnell, Ulrike Wittig, Roland Wohlgemuth, Carsten Kettner

Research output: Contribution to journalArticle

Abstract

A key component of enzyme function experiments is reporting of considerable metadata, to allow other researchers to replicate, interpret properly or use fully the results. This paper evaluates the completeness of enzyme function data reporting for reproducibility. We present a detailed examination of 11 recent papers (and their supplementary material) from two leading journals. We found that in every paper we were not able to collect some critical information necessary to reproduce the enzyme function findings. Study of 100 papers used by the SABIO-RK database confirmed some of the most common omissions: concentration of enzyme or its substrates, identity of counter-ions in buffers. A computer system should be better at preventing such omissions, helping secure the scientific record. Many of the omissions found would be trapped by the currently available version of STRENDA DB.
LanguageEnglish
Pages22-27
Number of pages6
JournalBiophysical Chemistry
Volume242
Early online date24 Aug 2018
DOIs
Publication statusPublished - 30 Nov 2018

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Enzymes
Radiation counters
Computer Systems
Metadata
Buffers
Computer systems
Research Design
Research Personnel
Databases
Ions
Substrates
Experiments

Keywords

  • enzyme function
  • metadata
  • reproducubility
  • transparency
  • data quality
  • standardization

Cite this

Halling, Peter ; Fitzpatrick, Paul F. ; Raushel, Frank M. ; Rohwer, Johann ; Schnell, Santiago ; Wittig, Ulrike ; Wohlgemuth, Roland ; Kettner, Carsten. / An empirical analysis of enzyme function reporting for experimental reproducibility : missing/incomplete information in published papers. In: Biophysical Chemistry. 2018 ; Vol. 242. pp. 22-27.
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An empirical analysis of enzyme function reporting for experimental reproducibility : missing/incomplete information in published papers. / Halling, Peter; Fitzpatrick, Paul F.; Raushel, Frank M.; Rohwer, Johann; Schnell, Santiago; Wittig, Ulrike; Wohlgemuth, Roland; Kettner, Carsten.

In: Biophysical Chemistry, Vol. 242, 30.11.2018, p. 22-27.

Research output: Contribution to journalArticle

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