TY - JOUR
T1 - An African Salmonella Typhimurium ST313 sublineage with extensive drug-resistance and signatures of host adaptation
AU - Van Puyvelde, Sandra
AU - Pickard, Derek
AU - Vandelannoote, Koen
AU - Heinz, Eva
AU - Barbé, Barbara
AU - de Block, Tessa
AU - Clare, Simon
AU - Coomber, Eve L.
AU - Harcourt, Katherine
AU - Sridhar, Sushmita
AU - Lees, Emily A.
AU - Wheeler, Nicole E.
AU - Klemm, Elizabeth J.
AU - Kuijpers, Laura
AU - Mbuyi Kalonji, Lisette
AU - Phoba, Marie France
AU - Falay, Dadi
AU - Ngbonda, Dauly
AU - Lunguya, Octavie
AU - Jacobs, Jan
AU - Dougan, Gordon
AU - Deborggraeve, Stijn
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Bloodstream infections by Salmonella enterica serovar Typhimurium constitute a major health burden in sub-Saharan Africa (SSA). These invasive non-typhoidal (iNTS) infections are dominated by isolates of the antibiotic resistance-associated sequence type (ST) 313. Here, we report emergence of ST313 sublineage II.1 in the Democratic Republic of the Congo. Sublineage II.1 exhibits extensive drug resistance, involving a combination of multidrug resistance, extended spectrum β-lactamase production and azithromycin resistance. ST313 lineage II.1 isolates harbour an IncHI2 plasmid we name pSTm-ST313-II.1, with one isolate also exhibiting decreased ciprofloxacin susceptibility. Whole genome sequencing reveals that ST313 II.1 isolates have accumulated genetic signatures potentially associated with altered pathogenicity and host adaptation, related to changes observed in biofilm formation and metabolic capacity. Sublineage II.1 emerged at the beginning of the 21st century and is involved in on-going outbreaks. Our data provide evidence of further evolution within the ST313 clade associated with iNTS in SSA.
AB - Bloodstream infections by Salmonella enterica serovar Typhimurium constitute a major health burden in sub-Saharan Africa (SSA). These invasive non-typhoidal (iNTS) infections are dominated by isolates of the antibiotic resistance-associated sequence type (ST) 313. Here, we report emergence of ST313 sublineage II.1 in the Democratic Republic of the Congo. Sublineage II.1 exhibits extensive drug resistance, involving a combination of multidrug resistance, extended spectrum β-lactamase production and azithromycin resistance. ST313 lineage II.1 isolates harbour an IncHI2 plasmid we name pSTm-ST313-II.1, with one isolate also exhibiting decreased ciprofloxacin susceptibility. Whole genome sequencing reveals that ST313 II.1 isolates have accumulated genetic signatures potentially associated with altered pathogenicity and host adaptation, related to changes observed in biofilm formation and metabolic capacity. Sublineage II.1 emerged at the beginning of the 21st century and is involved in on-going outbreaks. Our data provide evidence of further evolution within the ST313 clade associated with iNTS in SSA.
KW - Salmonella Typhimurium
KW - gastrointestinal infections
KW - bloodstream infections
UR - http://www.scopus.com/inward/record.url?scp=85072401116&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-11844-z
DO - 10.1038/s41467-019-11844-z
M3 - Article
C2 - 31537784
AN - SCOPUS:85072401116
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 4280
ER -