Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor

Wireeya Chawjiraphan; Chayachon Apiwat; Khoonsake Segkhoonthod; Kiatnida Treerattrakoon; Preedee Pinpradup; Nuankanya Sathirapongsasuti; Prapasiri Pongprayoon; Patraporn Luksirikul; Patcharee Isarankura-Na-Ayudhya; Deanpen Japrung

doi:10.1016/j.mex.2020.101114

Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor

Wireeya Chawjiraphan, Chayachon Apiwat, Khoonsake Segkhoonthod, Kiatnida Treerattrakoon, Preedee Pinpradup, Nuankanya Sathirapongsasuti, Prapasiri Pongprayoon, Patraporn Luksirikul, Patcharee Isarankura-Na-Ayudhya, Deanpen Japrung^*

^*Corresponding author for this work

Pure And Applied Chemistry

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Abstract

A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL⁻¹ and the detection range is 0.1–600 µg.mL⁻¹, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample. The limit of detection of our developed platform is 0.05 µg.mL^−1.The detection range of our aptasensor is 0.1–600 µg.mL⁻¹

Original language	English
Article number	101114
Number of pages	6
Journal	MethodsX
Volume	7
DOIs	https://doi.org/10.1016/j.mex.2020.101114
Publication status	Published - 20 Oct 2020

Funding

This work was supported by the National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand [grant number P1751330 ]. We also acknowledge the Research Network NANOTEC program (RNN-Ramathibodi hospital and RNN-Kasetsart University).

Keywords

albuminuria
albuminuria detection by graphene oxide-mediated fluorescence quenching aptasensor
aptasensor
chronic kidney disease
fluorescence quenching
graphene oxide
human serum albumin

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10.1016/j.mex.2020.101114Licence: CC BY 4.0

Chawjiraphan-etal-MX-2020-albuminuria-detection-using-graphene-oxide-mediated-fluorescenceFinal published version, 457 KBLicence: CC BY 4.0

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title = "Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor",

abstract = "A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample. The limit of detection of our developed platform is 0.05 µg.mL−1. The detection range of our aptasensor is 0.1–600 µg.mL−1",

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AU - Chawjiraphan, Wireeya

AU - Apiwat, Chayachon

AU - Segkhoonthod, Khoonsake

AU - Treerattrakoon, Kiatnida

AU - Pinpradup, Preedee

AU - Sathirapongsasuti, Nuankanya

AU - Pongprayoon, Prapasiri

AU - Luksirikul, Patraporn

AU - Isarankura-Na-Ayudhya, Patcharee

AU - Japrung, Deanpen

PY - 2020/10/20

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N2 - A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample. The limit of detection of our developed platform is 0.05 µg.mL−1. The detection range of our aptasensor is 0.1–600 µg.mL−1

AB - A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample. The limit of detection of our developed platform is 0.05 µg.mL−1. The detection range of our aptasensor is 0.1–600 µg.mL−1

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Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor

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