TY - JOUR
T1 - Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
AU - Chawjiraphan, Wireeya
AU - Apiwat, Chayachon
AU - Segkhoonthod, Khoonsake
AU - Treerattrakoon, Kiatnida
AU - Pinpradup, Preedee
AU - Sathirapongsasuti, Nuankanya
AU - Pongprayoon, Prapasiri
AU - Luksirikul, Patraporn
AU - Isarankura-Na-Ayudhya, Patcharee
AU - Japrung, Deanpen
PY - 2020/10/20
Y1 - 2020/10/20
N2 - A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample. The limit of detection of our developed platform is 0.05 µg.mL−1. The detection range of our aptasensor is 0.1–600 µg.mL−1
AB - A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample. The limit of detection of our developed platform is 0.05 µg.mL−1. The detection range of our aptasensor is 0.1–600 µg.mL−1
KW - albuminuria
KW - albuminuria detection by graphene oxide-mediated fluorescence quenching aptasensor
KW - aptasensor
KW - chronic kidney disease
KW - fluorescence quenching
KW - graphene oxide
KW - human serum albumin
UR - http://www.scopus.com/inward/record.url?scp=85094575071&partnerID=8YFLogxK
U2 - 10.1016/j.mex.2020.101114
DO - 10.1016/j.mex.2020.101114
M3 - Article
C2 - 32066078
AN - SCOPUS:85094575071
SN - 2215-0161
VL - 7
JO - MethodsX
JF - MethodsX
M1 - 101114
ER -