Agonist-evoked Ca2+ wave progression requires Ca2+ and IP3

J.G. McCarron, S. Chalmers, D. MacMillan, M.L. Olson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Smooth muscle responds to IP3-generating agonists by producing Ca2+ waves. Here, the mechanism of wave progression has been investigated in voltage-clamped single smooth muscle cells using localized photolysis of caged IP3 and the caged Ca2+ buffer diazo-2. Waves, evoked by the IP3-generating agonist carbachol (CCh), initiated as a uniform rise in cytoplasmic Ca2+ concentration ([Ca2+]c) over a single though substantial length (∼30 µm) of the cell. During regenerative propagation, the wave-front was about 1/3 the length (∼9 µm) of the initiation site. The wave-front progressed at a relatively constant velocity although amplitude varied through the cell; differences in sensitivity to IP3 may explain the amplitude changes. Ca2+ was required for IP3-mediated wave progression to occur. Increasing the Ca2+ buffer capacity in a small (2 µm) region immediately in front of a CCh-evoked Ca2+ wave halted progression at the site. However, the wave front does not progress by Ca2+-dependent positive feedback alone. In support, colliding [Ca2+]c increases from locally released IP3 did not annihilate but approximately doubled in amplitude. This result suggests that local IP3-evoked [Ca2+]c increases diffused passively. Failure of local increases in IP3 to evoke waves appears to arise from the restricted nature of the IP3 increase. When IP3 was elevated throughout the cell, a localized increase in Ca2+ now propagated as a wave. Together, these results suggest that waves initiate over a surprisingly large length of the cell and that both IP3 and Ca2+ are required for active propagation of the wave front to occur.
LanguageEnglish
Pages334-344
Number of pages11
JournalJournal of Cellular Physiology
Volume224
Issue number2
DOIs
Publication statusPublished - Aug 2010

Fingerprint

Carbachol
Buffers
Photolysis
Smooth Muscle Myocytes
Smooth Muscle
Muscle
Cells
Feedback
Electric potential
inositol 1,4,5-trisphosphate 1-(2-nitrophenyl)ethyl ester
diazo-2

Keywords

  • Ca2+ waves
  • wave progession
  • smooth muscle

Cite this

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title = "Agonist-evoked Ca2+ wave progression requires Ca2+ and IP3",
abstract = "Smooth muscle responds to IP3-generating agonists by producing Ca2+ waves. Here, the mechanism of wave progression has been investigated in voltage-clamped single smooth muscle cells using localized photolysis of caged IP3 and the caged Ca2+ buffer diazo-2. Waves, evoked by the IP3-generating agonist carbachol (CCh), initiated as a uniform rise in cytoplasmic Ca2+ concentration ([Ca2+]c) over a single though substantial length (∼30 µm) of the cell. During regenerative propagation, the wave-front was about 1/3 the length (∼9 µm) of the initiation site. The wave-front progressed at a relatively constant velocity although amplitude varied through the cell; differences in sensitivity to IP3 may explain the amplitude changes. Ca2+ was required for IP3-mediated wave progression to occur. Increasing the Ca2+ buffer capacity in a small (2 µm) region immediately in front of a CCh-evoked Ca2+ wave halted progression at the site. However, the wave front does not progress by Ca2+-dependent positive feedback alone. In support, colliding [Ca2+]c increases from locally released IP3 did not annihilate but approximately doubled in amplitude. This result suggests that local IP3-evoked [Ca2+]c increases diffused passively. Failure of local increases in IP3 to evoke waves appears to arise from the restricted nature of the IP3 increase. When IP3 was elevated throughout the cell, a localized increase in Ca2+ now propagated as a wave. Together, these results suggest that waves initiate over a surprisingly large length of the cell and that both IP3 and Ca2+ are required for active propagation of the wave front to occur.",
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Agonist-evoked Ca2+ wave progression requires Ca2+ and IP3. / McCarron, J.G.; Chalmers, S.; MacMillan, D.; Olson, M.L.

In: Journal of Cellular Physiology, Vol. 224, No. 2, 08.2010, p. 334-344.

Research output: Contribution to journalArticle

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