Aging and Ca2+ signaling in murine mesenteric arterial myocytes

J.E. Angermann, C. Mcallister, C. del Corsso, A.M. Gurney, S.M. Wilson

    Research output: Contribution to journalArticle

    Abstract

    Vascular reactivity may be reduced in old individuals and the (Ca2+)i is important to smooth muscle contractility. Experiments were therefore performed to determine if Ca2+ signaling is altered in mesenteric arterial smooth muscle cells (MASMCs) from 5-6 (young (Y)) and 29-30 month (old (O)) C57bl/6 mice. (Ca2+)i was measured using fura-2 imaging of individual MASMCs. The basal (Ca2+)i of MASMCs from Y (36+/-6 nM n=48) and O (51+/-4 nM n=101) were not significantly different. Total cytosolic Ca2+ (CaT) released by 30s 10 mM caffeine (CAF) or 10 ?M phenylephrine (PE) exposures were estimated by integrating the Ca2+ transient. CaT was proportional to peak (Ca2+)i in both Y and O, but the slope of CaT to peak (Ca2+)i was 2-fold greater in Y for both CAF and PE. The (Ca2+)i increase above basal following SR Ca2+ depletion in Y 66+/-8 nM n=19 was greater than in O 18+/-3 nM n=34. Following store depletion the (Ca2+)i clearance rate (Mclear) with extracellular Ca2+ removal in Y 0.0073+/-0.0009 s-1 n=19 was slower than in O 0.0161+/-0.0008 s-1 n=34. The (Ca2+)i cleared from the cytosol (Mclear*CaT) was proportional to the peak (Ca2+)i and was the same between Y and O for CAF, while that due to PE was greater in O. Aging modifies agonist-induced cytosolic Ca2+ increases and our data imply that altered cytosolic Ca2+ clearance mechanisms are an important component to the Ca2+ signaling impairments. NIH P20 RR15581 from NCRR, AG20400, HL10476, AI55462.
    LanguageEnglish
    PagesA703-A703
    JournalFASEB Journal
    Volume18
    Issue number4-5
    Publication statusPublished - 23 Mar 2004

    Fingerprint

    Phenylephrine
    Caffeine
    Muscle Cells
    Smooth Muscle Myocytes
    Muscle
    Aging of materials
    Fura-2
    Cytosol
    Smooth Muscle
    Blood Vessels
    Cells
    Imaging techniques
    Experiments

    Keywords

    • aging
    • cardiovascular system
    • circulatory
    • mesenteric arterial myocytes

    Cite this

    Angermann, J. E., Mcallister, C., del Corsso, C., Gurney, A. M., & Wilson, S. M. (2004). Aging and Ca2+ signaling in murine mesenteric arterial myocytes. FASEB Journal, 18(4-5), A703-A703.
    Angermann, J.E. ; Mcallister, C. ; del Corsso, C. ; Gurney, A.M. ; Wilson, S.M. / Aging and Ca2+ signaling in murine mesenteric arterial myocytes. In: FASEB Journal. 2004 ; Vol. 18, No. 4-5. pp. A703-A703.
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    Angermann, JE, Mcallister, C, del Corsso, C, Gurney, AM & Wilson, SM 2004, 'Aging and Ca2+ signaling in murine mesenteric arterial myocytes' FASEB Journal, vol. 18, no. 4-5, pp. A703-A703.

    Aging and Ca2+ signaling in murine mesenteric arterial myocytes. / Angermann, J.E.; Mcallister, C.; del Corsso, C.; Gurney, A.M.; Wilson, S.M.

    In: FASEB Journal, Vol. 18, No. 4-5, 23.03.2004, p. A703-A703.

    Research output: Contribution to journalArticle

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    Angermann JE, Mcallister C, del Corsso C, Gurney AM, Wilson SM. Aging and Ca2+ signaling in murine mesenteric arterial myocytes. FASEB Journal. 2004 Mar 23;18(4-5):A703-A703.