Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor

P. Besenius, P.A.G. Cormack, R.F. Ludlow, S.R. Otto, D.C. Sherrington

Research output: Contribution to journalArticle

24 Citations (Scopus)
92 Downloads (Pure)

Abstract

We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly(N,N-dimethylacrylamide)-based support, unselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular recognition arising in aqueous solution reactions is manifest also in the analogous templated solid phase DCL syntheses. The ability of a polymer bound template to identify and isolate a synthetic receptor via dynamic combinatorial chemistry was not compromised by the large size of the library, consisting of well over 140 theoretical members, demonstrating the practical advantages of a polymer-supported DCL methodology.
Original languageEnglish
Pages (from-to)2414-2418
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume8
Issue number10
DOIs
Publication statusPublished - 2010

Keywords

  • acrylamides
  • amantadines
  • chromatography
  • high pressure liquid
  • nanostructures

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