Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording

L. Breckenridge, R.J.A. Wilson, P. Connolly, A.S.G. Curtis, J.A.T. Dow, E. Blackshaw, C.D.W. Wilkinson

    Research output: Contribution to journalArticle

    79 Citations (Scopus)

    Abstract

    We describe fabrication methods and the characterisation and use of extracellalar microelectrode arrays for the detection of action potentials from neurons in culture. The 100 μm2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action potential with S:N ratios of up to 500:1, giving a maximum recorded signal of several millivolts. The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non-invasive nature, high packing density, and utility in stimulation, are demonstrated.
    LanguageEnglish
    Pages266-276
    Number of pages10
    JournalJournal of Neuroscience Research
    Volume42
    Issue number2
    Publication statusPublished - 1995

    Fingerprint

    Electrodes
    Microelectrodes
    Neurons
    Action Potentials
    Invertebrates
    In Vitro Techniques
    Gold

    Keywords

    • extracellular microelectrode arrays
    • multisite recording
    • cell/electrode seal
    • neuronal networks

    Cite this

    Breckenridge, L., Wilson, R. J. A., Connolly, P., Curtis, A. S. G., Dow, J. A. T., Blackshaw, E., & Wilkinson, C. D. W. (1995). Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording. Journal of Neuroscience Research, 42(2), 266-276.
    Breckenridge, L. ; Wilson, R.J.A. ; Connolly, P. ; Curtis, A.S.G. ; Dow, J.A.T. ; Blackshaw, E. ; Wilkinson, C.D.W. / Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording. In: Journal of Neuroscience Research. 1995 ; Vol. 42, No. 2. pp. 266-276.
    @article{e0a1e3fa15d148d1932a2d21b1d4869b,
    title = "Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording",
    abstract = "We describe fabrication methods and the characterisation and use of extracellalar microelectrode arrays for the detection of action potentials from neurons in culture. The 100 μm2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action potential with S:N ratios of up to 500:1, giving a maximum recorded signal of several millivolts. The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non-invasive nature, high packing density, and utility in stimulation, are demonstrated.",
    keywords = "extracellular microelectrode arrays, multisite recording, cell/electrode seal, neuronal networks",
    author = "L. Breckenridge and R.J.A. Wilson and P. Connolly and A.S.G. Curtis and J.A.T. Dow and E. Blackshaw and C.D.W. Wilkinson",
    year = "1995",
    language = "English",
    volume = "42",
    pages = "266--276",
    journal = "Journal of Neuroscience Research",
    issn = "0360-4012",
    number = "2",

    }

    Breckenridge, L, Wilson, RJA, Connolly, P, Curtis, ASG, Dow, JAT, Blackshaw, E & Wilkinson, CDW 1995, 'Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording' Journal of Neuroscience Research, vol. 42, no. 2, pp. 266-276.

    Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording. / Breckenridge, L.; Wilson, R.J.A.; Connolly, P.; Curtis, A.S.G.; Dow, J.A.T.; Blackshaw, E.; Wilkinson, C.D.W.

    In: Journal of Neuroscience Research, Vol. 42, No. 2, 1995, p. 266-276.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording

    AU - Breckenridge, L.

    AU - Wilson, R.J.A.

    AU - Connolly, P.

    AU - Curtis, A.S.G.

    AU - Dow, J.A.T.

    AU - Blackshaw, E.

    AU - Wilkinson, C.D.W.

    PY - 1995

    Y1 - 1995

    N2 - We describe fabrication methods and the characterisation and use of extracellalar microelectrode arrays for the detection of action potentials from neurons in culture. The 100 μm2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action potential with S:N ratios of up to 500:1, giving a maximum recorded signal of several millivolts. The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non-invasive nature, high packing density, and utility in stimulation, are demonstrated.

    AB - We describe fabrication methods and the characterisation and use of extracellalar microelectrode arrays for the detection of action potentials from neurons in culture. The 100 μm2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action potential with S:N ratios of up to 500:1, giving a maximum recorded signal of several millivolts. The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non-invasive nature, high packing density, and utility in stimulation, are demonstrated.

    KW - extracellular microelectrode arrays

    KW - multisite recording

    KW - cell/electrode seal

    KW - neuronal networks

    UR - http://dx/doi.org/10.1002/jnr.490420215

    M3 - Article

    VL - 42

    SP - 266

    EP - 276

    JO - Journal of Neuroscience Research

    T2 - Journal of Neuroscience Research

    JF - Journal of Neuroscience Research

    SN - 0360-4012

    IS - 2

    ER -

    Breckenridge L, Wilson RJA, Connolly P, Curtis ASG, Dow JAT, Blackshaw E et al. Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording. Journal of Neuroscience Research. 1995;42(2):266-276.