Advances in super-resolution stimulated Raman scattering microscopy

William J. Tipping*, Karen Faulds, Duncan Graham*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Super-resolution optical imaging overcomes the diffraction limit in light microscopy to enable the visualization of previously invisible molecular details within a sample. The realization of super-resolution imaging based on stimulated Raman scattering (SRS) microscopy represents a recent area of fruitful development that has been used to visualize cellular structures in three dimensions, with multiple spectroscopic colors at the nanometer scale. Several fundamental approaches to achieving super-resolution SRS imaging have been reported, including optical engineering strategies, expansion microscopy, deconvolution image analysis, and photo switchable SRS reporters as methods to break the diffraction limit. These approaches have enabled the visualization of biological structures, cellular interactions, and dynamics with unprecedented detail. In this Perspective, an overview of the current strategies and capabilities for achieving super-resolution SRS imaging will be highlighted together with an outlook on potential directions of this rapidly evolving field.
Original languageEnglish
Pages (from-to)733-743
Number of pages11
JournalChemical & Biomedical Imaging
Volume2
Issue number11
Early online date4 Oct 2024
DOIs
Publication statusPublished - 25 Nov 2024

Keywords

  • stimulated Raman scattering microscopy
  • structure−function relationships,
  • uper-resolution imaging
  • photoswitchable probes

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