Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy

D. D-U Li; S. Poland; S. Coelho; D. Tyndall; W. Zhang; J. Richardson; R. K. Henderson; S. M. Ameer-Beg

doi:10.1109/EMBC.2013.6610180

Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy

D. D-U Li, S. Poland, S. Coelho, D. Tyndall, W. Zhang, J. Richardson, R. K. Henderson, S. M. Ameer-Beg

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

Abstract

We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.

Original language	English
Title of host publication	35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
Publisher	IEEE
Pages	3036-3039
Number of pages	4
ISBN (Print)	9781457702167
DOIs	https://doi.org/10.1109/EMBC.2013.6610180
Publication status	Published - 3 Jul 2013
Event	35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Osaka International Convention Center, Osaka, Japan Duration: 3 Jul 2013 → 7 Jul 2013 http://embc2013.embs.org/

Publication series

Name
ISSN (Print)	1094-687X
ISSN (Electronic)	1558-4615

Conference

Conference	35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Abbreviated title	EMBC’13
Country/Territory	Japan
City	Osaka
Period	3/07/13 → 7/07/13
Internet address	http://embc2013.embs.org/

Keywords

optical microscopy
avalanche diodes
biomedical optical imaging
CMOS image sensors
fluorescence
multibeam multiphoton microscopy system
fluorescence lifetime imaging algorithms
CMOS single-photon sensor
multifocal multiphoton microscopy system
biexponential fluorescence lifetime algorithm
2D CMOS single-photon avalanche diode arrays
plant specimen
CMOS SPAD arrays

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10.1109/EMBC.2013.6610180

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Li, D. D.-U., Poland, S., Coelho, S., Tyndall, D., Zhang, W., Richardson, J., Henderson, R. K., & Ameer-Beg, S. M. (2013). Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. In 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) (pp. 3036-3039). IEEE. https://doi.org/10.1109/EMBC.2013.6610180

@inproceedings{31f11f5d98a6422696c4bb8a5f36d61b,

title = "Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy",

abstract = "We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.",

keywords = "optical microscopy, avalanche diodes, biomedical optical imaging, CMOS image sensors, fluorescence, multibeam multiphoton microscopy system, fluorescence lifetime imaging algorithms, CMOS single-photon sensor, multifocal multiphoton microscopy system, biexponential fluorescence lifetime algorithm, 2D CMOS single-photon avalanche diode arrays, plant specimen, CMOS SPAD arrays",

author = "Li, {D. D-U} and S. Poland and S. Coelho and D. Tyndall and W. Zhang and J. Richardson and Henderson, {R. K.} and Ameer-Beg, {S. M.}",

year = "2013",

month = jul,

day = "3",

doi = "10.1109/EMBC.2013.6610180",

language = "English",

isbn = "9781457702167",

publisher = "IEEE",

pages = "3036--3039",

booktitle = "35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)",

note = "35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC{\textquoteright}13 ; Conference date: 03-07-2013 Through 07-07-2013",

url = "http://embc2013.embs.org/",

}

Li, DD-U, Poland, S, Coelho, S, Tyndall, D, Zhang, W, Richardson, J, Henderson, RK & Ameer-Beg, SM 2013, Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. in 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, pp. 3036-3039, 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Osaka, Japan, 3/07/13. https://doi.org/10.1109/EMBC.2013.6610180

Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. / Li, D. D-U; Poland, S.; Coelho, S. et al.
35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. p. 3036-3039.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

TY - GEN

T1 - Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy

AU - Li, D. D-U

AU - Poland, S.

AU - Coelho, S.

AU - Tyndall, D.

AU - Zhang, W.

AU - Richardson, J.

AU - Henderson, R. K.

AU - Ameer-Beg, S. M.

PY - 2013/7/3

Y1 - 2013/7/3

N2 - We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.

AB - We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.

KW - optical microscopy

KW - avalanche diodes

KW - biomedical optical imaging

KW - CMOS image sensors

KW - fluorescence

KW - multibeam multiphoton microscopy system

KW - fluorescence lifetime imaging algorithms

KW - CMOS single-photon sensor

KW - multifocal multiphoton microscopy system

KW - biexponential fluorescence lifetime algorithm

KW - 2D CMOS single-photon avalanche diode arrays

KW - plant specimen

KW - CMOS SPAD arrays

UR - http://embc2013.embs.org/

U2 - 10.1109/EMBC.2013.6610180

DO - 10.1109/EMBC.2013.6610180

M3 - Conference contribution book

SN - 9781457702167

SP - 3036

EP - 3039

BT - 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

PB - IEEE

T2 - 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Y2 - 3 July 2013 through 7 July 2013

ER -

Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy

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