Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy

D. D-U Li, S. Poland, S. Coelho, D. Tyndall, W. Zhang, J. Richardson, R. K. Henderson, S. M. Ameer-Beg

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.
LanguageEnglish
Title of host publication35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
PublisherIEEE
Pages3036-3039
Number of pages4
ISBN (Print)9781457702167
DOIs
Publication statusPublished - 3 Jul 2013
Event35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Osaka International Convention Center, Osaka, Japan
Duration: 3 Jul 20137 Jul 2013
http://embc2013.embs.org/

Publication series

Name
ISSN (Print)1094-687X
ISSN (Electronic)1558-4615

Conference

Conference35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Abbreviated titleEMBC’13
CountryJapan
CityOsaka
Period3/07/137/07/13
Internet address

Fingerprint

CMOS
avalanche diodes
microscopy
life (durability)
fluorescence
sensors
photons
hardware

Keywords

  • optical microscopy
  • avalanche diodes
  • biomedical optical imaging
  • CMOS image sensors
  • fluorescence
  • multibeam multiphoton microscopy system
  • fluorescence lifetime imaging algorithms
  • CMOS single-photon sensor
  • multifocal multiphoton microscopy system
  • biexponential fluorescence lifetime algorithm
  • 2D CMOS single-photon avalanche diode arrays
  • plant specimen
  • CMOS SPAD arrays

Cite this

Li, D. D-U., Poland, S., Coelho, S., Tyndall, D., Zhang, W., Richardson, J., ... Ameer-Beg, S. M. (2013). Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. In 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) (pp. 3036-3039). IEEE. https://doi.org/10.1109/EMBC.2013.6610180
Li, D. D-U ; Poland, S. ; Coelho, S. ; Tyndall, D. ; Zhang, W. ; Richardson, J. ; Henderson, R. K. ; Ameer-Beg, S. M. / Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. pp. 3036-3039
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title = "Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy",
abstract = "We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.",
keywords = "optical microscopy, avalanche diodes, biomedical optical imaging, CMOS image sensors, fluorescence, multibeam multiphoton microscopy system, fluorescence lifetime imaging algorithms, CMOS single-photon sensor, multifocal multiphoton microscopy system, biexponential fluorescence lifetime algorithm, 2D CMOS single-photon avalanche diode arrays, plant specimen, CMOS SPAD arrays",
author = "Li, {D. D-U} and S. Poland and S. Coelho and D. Tyndall and W. Zhang and J. Richardson and Henderson, {R. K.} and Ameer-Beg, {S. M.}",
year = "2013",
month = "7",
day = "3",
doi = "10.1109/EMBC.2013.6610180",
language = "English",
isbn = "9781457702167",
publisher = "IEEE",
pages = "3036--3039",
booktitle = "35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)",

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Li, DD-U, Poland, S, Coelho, S, Tyndall, D, Zhang, W, Richardson, J, Henderson, RK & Ameer-Beg, SM 2013, Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. in 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, pp. 3036-3039, 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Osaka, Japan, 3/07/13. https://doi.org/10.1109/EMBC.2013.6610180

Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. / Li, D. D-U; Poland, S.; Coelho, S.; Tyndall, D.; Zhang, W.; Richardson, J.; Henderson, R. K.; Ameer-Beg, S. M.

35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. p. 3036-3039.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

TY - GEN

T1 - Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy

AU - Li, D. D-U

AU - Poland, S.

AU - Coelho, S.

AU - Tyndall, D.

AU - Zhang, W.

AU - Richardson, J.

AU - Henderson, R. K.

AU - Ameer-Beg, S. M.

PY - 2013/7/3

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N2 - We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.

AB - We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.

KW - optical microscopy

KW - avalanche diodes

KW - biomedical optical imaging

KW - CMOS image sensors

KW - fluorescence

KW - multibeam multiphoton microscopy system

KW - fluorescence lifetime imaging algorithms

KW - CMOS single-photon sensor

KW - multifocal multiphoton microscopy system

KW - biexponential fluorescence lifetime algorithm

KW - 2D CMOS single-photon avalanche diode arrays

KW - plant specimen

KW - CMOS SPAD arrays

UR - http://embc2013.embs.org/

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DO - 10.1109/EMBC.2013.6610180

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BT - 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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Li DD-U, Poland S, Coelho S, Tyndall D, Zhang W, Richardson J et al. Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. In 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE. 2013. p. 3036-3039 https://doi.org/10.1109/EMBC.2013.6610180