Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy

D. D-U Li, S. Poland, S. Coelho, D. Tyndall, W. Zhang, J. Richardson, R. K. Henderson, S. M. Ameer-Beg

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13μm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.
Original languageEnglish
Title of host publication35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
PublisherIEEE
Pages3036-3039
Number of pages4
ISBN (Print)9781457702167
DOIs
Publication statusPublished - 3 Jul 2013
Event35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Osaka International Convention Center, Osaka, Japan
Duration: 3 Jul 20137 Jul 2013
http://embc2013.embs.org/

Publication series

Name
ISSN (Print)1094-687X
ISSN (Electronic)1558-4615

Conference

Conference35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Abbreviated titleEMBC’13
CountryJapan
CityOsaka
Period3/07/137/07/13
Internet address

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Keywords

  • optical microscopy
  • avalanche diodes
  • biomedical optical imaging
  • CMOS image sensors
  • fluorescence
  • multibeam multiphoton microscopy system
  • fluorescence lifetime imaging algorithms
  • CMOS single-photon sensor
  • multifocal multiphoton microscopy system
  • biexponential fluorescence lifetime algorithm
  • 2D CMOS single-photon avalanche diode arrays
  • plant specimen
  • CMOS SPAD arrays

Cite this

Li, D. D-U., Poland, S., Coelho, S., Tyndall, D., Zhang, W., Richardson, J., ... Ameer-Beg, S. M. (2013). Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy. In 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) (pp. 3036-3039). IEEE. https://doi.org/10.1109/EMBC.2013.6610180