Administering labelled water to exclusively breast-fed infants in studies involving stable isotope techniques

S.B. Nielsen, J.C. Wells, C. Slater, M.S. Fewtrell, John J Reilly

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Stable isotope techniques using deuterium or 18O are reference methods for assessing total body water (TBW) for body composition. In combination, they provide total energy expenditure and human milk intake in exclusively breast-fed (EBF) infants. These techniques require an oral administration of an accurately quantified dose of labelled water to infants, who often have no prior experience of consuming water. In the present study, 18O labelled water was administered to 47 EBF infants at two time points. Route of administration, duration of dosing and spills were quantified and recorded. Eighty-seven out of 94 (92.6%) dose administrations were successful. In two-thirds of dose administrations,>90% of the prepared dose was consumed. Spills ranged from 0.2% to 57%. Approaches to correct for spills can introduce bias in the calculation of TBW. Minimising and recording all dose spills is an important issue for the accuracy and precision of stable isotope techniques, when applied to EBF infants.

LanguageEnglish
Pages18-25
Number of pages8
JournalIsotopes in Environmental and Health Studies
Volume47
Issue number1
DOIs
Publication statusPublished - Mar 2011

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Isotopes
Hazardous materials spills
stable isotope
Water
water
Deuterium
deuterium
milk
expenditure
dose
infant
Chemical analysis
energy
water body

Keywords

  • body composition
  • breast-fed infants
  • doubly-labelled water
  • total body water
  • stable isotope tracer techniques

Cite this

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abstract = "Stable isotope techniques using deuterium or 18O are reference methods for assessing total body water (TBW) for body composition. In combination, they provide total energy expenditure and human milk intake in exclusively breast-fed (EBF) infants. These techniques require an oral administration of an accurately quantified dose of labelled water to infants, who often have no prior experience of consuming water. In the present study, 18O labelled water was administered to 47 EBF infants at two time points. Route of administration, duration of dosing and spills were quantified and recorded. Eighty-seven out of 94 (92.6{\%}) dose administrations were successful. In two-thirds of dose administrations,>90{\%} of the prepared dose was consumed. Spills ranged from 0.2{\%} to 57{\%}. Approaches to correct for spills can introduce bias in the calculation of TBW. Minimising and recording all dose spills is an important issue for the accuracy and precision of stable isotope techniques, when applied to EBF infants.",
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Administering labelled water to exclusively breast-fed infants in studies involving stable isotope techniques. / Nielsen, S.B.; Wells, J.C.; Slater, C.; Fewtrell, M.S.; Reilly, John J.

In: Isotopes in Environmental and Health Studies , Vol. 47, No. 1, 03.2011, p. 18-25.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Administering labelled water to exclusively breast-fed infants in studies involving stable isotope techniques

AU - Nielsen, S.B.

AU - Wells, J.C.

AU - Slater, C.

AU - Fewtrell, M.S.

AU - Reilly, John J

PY - 2011/3

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N2 - Stable isotope techniques using deuterium or 18O are reference methods for assessing total body water (TBW) for body composition. In combination, they provide total energy expenditure and human milk intake in exclusively breast-fed (EBF) infants. These techniques require an oral administration of an accurately quantified dose of labelled water to infants, who often have no prior experience of consuming water. In the present study, 18O labelled water was administered to 47 EBF infants at two time points. Route of administration, duration of dosing and spills were quantified and recorded. Eighty-seven out of 94 (92.6%) dose administrations were successful. In two-thirds of dose administrations,>90% of the prepared dose was consumed. Spills ranged from 0.2% to 57%. Approaches to correct for spills can introduce bias in the calculation of TBW. Minimising and recording all dose spills is an important issue for the accuracy and precision of stable isotope techniques, when applied to EBF infants.

AB - Stable isotope techniques using deuterium or 18O are reference methods for assessing total body water (TBW) for body composition. In combination, they provide total energy expenditure and human milk intake in exclusively breast-fed (EBF) infants. These techniques require an oral administration of an accurately quantified dose of labelled water to infants, who often have no prior experience of consuming water. In the present study, 18O labelled water was administered to 47 EBF infants at two time points. Route of administration, duration of dosing and spills were quantified and recorded. Eighty-seven out of 94 (92.6%) dose administrations were successful. In two-thirds of dose administrations,>90% of the prepared dose was consumed. Spills ranged from 0.2% to 57%. Approaches to correct for spills can introduce bias in the calculation of TBW. Minimising and recording all dose spills is an important issue for the accuracy and precision of stable isotope techniques, when applied to EBF infants.

KW - body composition

KW - breast-fed infants

KW - doubly-labelled water

KW - total body water

KW - stable isotope tracer techniques

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