Adaptive response to oxidative stress in the filamentous fungus Aspergillus niger B1-D

Qiang Li, Brian Mcneil, L.M. Harvey

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

In the present study, we used a recombinant filamentous fungus strain, Aspergillus niger B1-D, as a model system, and investigated the antioxidant defences in this organism. Our findings indicate that pretreatment with low concentrations of H2O2 completely prevents killing by this oxidant at high concentrations. It shows that A. niger adapts to exposure to H2O2 by reducing growth and inducing a number of antioxidant enzyme activities, including superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, of which the induction of catalase is the most pronounced. Moreover the decline of these antioxidant enzymes activities after H2O2 detoxification, coincides with recommencement of growth. Results from monitoring the extracellular H2O2 concentration clearly indicate a very rapid detoxification rate for H2O2 in adapted A. niger cultures. A mathematical model predicts only very low concentrations of intracellular H2O2 accumulating in such cultures. Our results also show that glutathione plays a role in the oxidative defence against H2O2 in A. niger. On addition of H2O2, the intracellular pool of glutathione increases while the redox state of glutathione becomes more oxidized.
LanguageEnglish
Pages394-402
Number of pages9
JournalFree Radical Biology and Medicine
Volume44
Issue number3
DOIs
Publication statusPublished - 1 Feb 2008

Fingerprint

Oxidative stress
Aspergillus niger
Aspergillus
Fungi
Glutathione
Detoxification
Oxidative Stress
Antioxidants
Enzyme activity
Catalase
Glutathione Reductase
Glutathione Peroxidase
Oxidants
Superoxide Dismutase
Enzymes
Growth
Mathematical models
Oxidation-Reduction
Theoretical Models
Monitoring

Keywords

  • oxidative stress
  • filamentous fungi
  • adaptive responses
  • H2O2
  • antioxidant enzymes

Cite this

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abstract = "In the present study, we used a recombinant filamentous fungus strain, Aspergillus niger B1-D, as a model system, and investigated the antioxidant defences in this organism. Our findings indicate that pretreatment with low concentrations of H2O2 completely prevents killing by this oxidant at high concentrations. It shows that A. niger adapts to exposure to H2O2 by reducing growth and inducing a number of antioxidant enzyme activities, including superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, of which the induction of catalase is the most pronounced. Moreover the decline of these antioxidant enzymes activities after H2O2 detoxification, coincides with recommencement of growth. Results from monitoring the extracellular H2O2 concentration clearly indicate a very rapid detoxification rate for H2O2 in adapted A. niger cultures. A mathematical model predicts only very low concentrations of intracellular H2O2 accumulating in such cultures. Our results also show that glutathione plays a role in the oxidative defence against H2O2 in A. niger. On addition of H2O2, the intracellular pool of glutathione increases while the redox state of glutathione becomes more oxidized.",
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Adaptive response to oxidative stress in the filamentous fungus Aspergillus niger B1-D. / Li, Qiang; Mcneil, Brian; Harvey, L.M.

In: Free Radical Biology and Medicine, Vol. 44, No. 3, 01.02.2008, p. 394-402.

Research output: Contribution to journalArticle

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