Adaptive optics in confocal and two-photon microscopy of rat brain: a single correction per optical section

J.M. Girkin, J. Vijverberg, M.A. Orazio, S.P. Poland, A. Wright, S. Cobb

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Sample induced optical aberrations in slices of rat brain tissue have been corrected with a deformable membrane mirror. The aberration correction required by the DMM was determined using a genetic algorithm with the intensity at a point in the sample as a fitness value. We show that by optimising on the intensity of a single point in the sample we are able to improve the axial resolution across the whole field of view of the image at a fixed sample depth. The ratio between the corrected axial resolution and the diffraction limited resolution is on average 2.7 for a 50 µm thick rat brain tissue sample and 12 for a 380 µm thick sample across the whole field of view. The uncorrected ratio being 4.1 and 15.5 respectively. Using a single aberration correction per depth, compared to a point-by-point aberration correction, will significantly decrease scan times and therefore reduce photobleaching and phototoxic effects enabling more rapid microscopy with active aberration correction.
LanguageEnglish
Pages64420T
JournalProceedings of SPIE: The International Society for Optical Engineering
Volume6442
DOIs
Publication statusPublished - 2007

Fingerprint

Adaptive optics
Confocal
Adaptive Optics
Aberrations
adaptive optics
Aberration Correction
Microscopy
rats
brain
Rats
Brain
Microscopic examination
Photon
Photons
aberration
microscopy
photons
Field of View
field of view
Tissue

Keywords

  • confocal microscopy
  • multiphoton microscopy
  • adaptive optics
  • aberration correction
  • spherical-aberration
  • mirror
  • implementation
  • system
  • water

Cite this

Girkin, J.M. ; Vijverberg, J. ; Orazio, M.A. ; Poland, S.P. ; Wright, A. ; Cobb, S. / Adaptive optics in confocal and two-photon microscopy of rat brain: a single correction per optical section. In: Proceedings of SPIE: The International Society for Optical Engineering. 2007 ; Vol. 6442. pp. 64420T.
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Adaptive optics in confocal and two-photon microscopy of rat brain: a single correction per optical section. / Girkin, J.M.; Vijverberg, J.; Orazio, M.A.; Poland, S.P.; Wright, A.; Cobb, S.

In: Proceedings of SPIE: The International Society for Optical Engineering, Vol. 6442, 2007, p. 64420T.

Research output: Contribution to journalArticle

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T1 - Adaptive optics in confocal and two-photon microscopy of rat brain: a single correction per optical section

AU - Girkin, J.M.

AU - Vijverberg, J.

AU - Orazio, M.A.

AU - Poland, S.P.

AU - Wright, A.

AU - Cobb, S.

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KW - spherical-aberration

KW - mirror

KW - implementation

KW - system

KW - water

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