Adaptive Optics for Fluorescence Microscopy

Martin J. Booth, Brian R. Patton

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The image quality of high-resolution fluorescence microscopes is often compromised by aberrations, particularly when they are used to focus deep into tissue specimens. The techniques of adaptive optics have been developed to overcome the problems caused by aberrations. The various components of an adaptive optical system are discussed alongside specific considerations that must be made in the design of adaptive fluorescence microscopes. Different implementations are outlined and the future of this technology is discussed.

Original languageEnglish
Title of host publicationFluorescence Microscopy
Subtitle of host publicationSuper-Resolution and other Novel Techniques
PublisherElsevier Inc.
Pages15-33
Number of pages19
ISBN (Electronic)9780124167131
ISBN (Print)9780124095137
DOIs
Publication statusPublished - 18 Mar 2014
Externally publishedYes

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Keywords

  • aberrations
  • adaptive optics
  • fluorescence
  • imaging
  • microscope

Cite this

Booth, M. J., & Patton, B. R. (2014). Adaptive Optics for Fluorescence Microscopy. In Fluorescence Microscopy: Super-Resolution and other Novel Techniques (pp. 15-33). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-409513-7.00002-6