Adaptive Optics for Fluorescence Microscopy

Martin J. Booth; Brian R. Patton

doi:10.1016/B978-0-12-409513-7.00002-6

Adaptive Optics for Fluorescence Microscopy

Martin J. Booth^*, Brian R. Patton

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

7 Citations (Scopus)

Abstract

The image quality of high-resolution fluorescence microscopes is often compromised by aberrations, particularly when they are used to focus deep into tissue specimens. The techniques of adaptive optics have been developed to overcome the problems caused by aberrations. The various components of an adaptive optical system are discussed alongside specific considerations that must be made in the design of adaptive fluorescence microscopes. Different implementations are outlined and the future of this technology is discussed.

Original language	English
Title of host publication	Fluorescence Microscopy
Subtitle of host publication	Super-Resolution and other Novel Techniques
Publisher	Elsevier Inc.
Pages	15-33
Number of pages	19
ISBN (Electronic)	9780124167131
ISBN (Print)	9780124095137
DOIs	https://doi.org/10.1016/B978-0-12-409513-7.00002-6
Publication status	Published - 18 Mar 2014
Externally published	Yes

Keywords

aberrations
adaptive optics
fluorescence
imaging
microscope

Access to Document

10.1016/B978-0-12-409513-7.00002-6

Cite this

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title = "Adaptive Optics for Fluorescence Microscopy",

abstract = "The image quality of high-resolution fluorescence microscopes is often compromised by aberrations, particularly when they are used to focus deep into tissue specimens. The techniques of adaptive optics have been developed to overcome the problems caused by aberrations. The various components of an adaptive optical system are discussed alongside specific considerations that must be made in the design of adaptive fluorescence microscopes. Different implementations are outlined and the future of this technology is discussed.",

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AU - Patton, Brian R.

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AB - The image quality of high-resolution fluorescence microscopes is often compromised by aberrations, particularly when they are used to focus deep into tissue specimens. The techniques of adaptive optics have been developed to overcome the problems caused by aberrations. The various components of an adaptive optical system are discussed alongside specific considerations that must be made in the design of adaptive fluorescence microscopes. Different implementations are outlined and the future of this technology is discussed.

KW - aberrations

KW - adaptive optics

KW - fluorescence

KW - imaging

KW - microscope

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M3 - Chapter

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SN - 9780124095137

SP - 15

EP - 33

BT - Fluorescence Microscopy

PB - Elsevier Inc.

ER -

Adaptive Optics for Fluorescence Microscopy

Abstract

Keywords

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