Adaptive Optics for Fluorescence Microscopy

Martin J. Booth, Brian R. Patton

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The image quality of high-resolution fluorescence microscopes is often compromised by aberrations, particularly when they are used to focus deep into tissue specimens. The techniques of adaptive optics have been developed to overcome the problems caused by aberrations. The various components of an adaptive optical system are discussed alongside specific considerations that must be made in the design of adaptive fluorescence microscopes. Different implementations are outlined and the future of this technology is discussed.

Original languageEnglish
Title of host publicationFluorescence Microscopy
Subtitle of host publicationSuper-Resolution and other Novel Techniques
Pages15-33
Number of pages19
ISBN (Electronic)9780124167131
DOIs
Publication statusPublished - 18 Mar 2014
Externally publishedYes

Fingerprint

Adaptive optics
Fluorescence microscopy
Aberrations
adaptive optics
Fluorescence Microscopy
aberration
Microscopes
Fluorescence
microscopes
microscopy
Optical Devices
fluorescence
Optical systems
Image quality
Tissue
Technology
high resolution

Keywords

  • aberrations
  • adaptive optics
  • fluorescence
  • imaging
  • microscope

Cite this

Booth, M. J., & Patton, B. R. (2014). Adaptive Optics for Fluorescence Microscopy. In Fluorescence Microscopy: Super-Resolution and other Novel Techniques (pp. 15-33) https://doi.org/10.1016/B978-0-12-409513-7.00002-6
Booth, Martin J. ; Patton, Brian R. / Adaptive Optics for Fluorescence Microscopy. Fluorescence Microscopy: Super-Resolution and other Novel Techniques. 2014. pp. 15-33
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Booth, MJ & Patton, BR 2014, Adaptive Optics for Fluorescence Microscopy. in Fluorescence Microscopy: Super-Resolution and other Novel Techniques. pp. 15-33. https://doi.org/10.1016/B978-0-12-409513-7.00002-6

Adaptive Optics for Fluorescence Microscopy. / Booth, Martin J.; Patton, Brian R.

Fluorescence Microscopy: Super-Resolution and other Novel Techniques. 2014. p. 15-33.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Booth MJ, Patton BR. Adaptive Optics for Fluorescence Microscopy. In Fluorescence Microscopy: Super-Resolution and other Novel Techniques. 2014. p. 15-33 https://doi.org/10.1016/B978-0-12-409513-7.00002-6