Adaptive optics for deeper imaging of biological samples

J.M. Girkin, S. Poland, A.J. Wright

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Optical microscopy has been a cornerstone of life science investigations since its first practical application around 400 years ago with the goal being subcellular resolution, three-dimensional images, at depth, in living samples. Nonlinear microscopy brought this dream a step closer, but as one images more deeply the material through which you image can greatly distort the view. By using optical devices, originally developed for astronomy, whose optical properties can be changed in real time, active compensation for sample-induced aberrations is possible. Submicron resolution images are now routinely recorded from depths over 1 mm into tissue. Such active optical elements can also be used to keep conventional microscopes, both confocal and widefield, in optimal alignment.
Original languageEnglish
Pages (from-to)106–110
Number of pages5
JournalCurrent Opinion in Biotechnology
Volume20
Issue number1
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Adaptive optics
Optical devices
Microscopy
Astronomy
Imaging techniques
Optical Devices
Three-Dimensional Imaging
Biological Science Disciplines
Image resolution
Aberrations
Optical microscopy
Microscopic examination
Microscopes
Optical properties
Tissue
Compensation and Redress

Keywords

  • adaptive optics
  • optics
  • imaging
  • photonics

Cite this

Girkin, J.M. ; Poland, S. ; Wright, A.J. / Adaptive optics for deeper imaging of biological samples. In: Current Opinion in Biotechnology. 2009 ; Vol. 20, No. 1. pp. 106–110.
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Adaptive optics for deeper imaging of biological samples. / Girkin, J.M.; Poland, S.; Wright, A.J.

In: Current Opinion in Biotechnology, Vol. 20, No. 1, 02.2009, p. 106–110.

Research output: Contribution to journalArticle

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