Adaptive optics correction of specimen-induced aberrations in single-molecule switching microscopy

Daniel Burke, Brian Patton, Fang Huang, Joerg Bewersdorf, Martin J. Booth

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Single-molecule switching (SMS) microscopy is a super-resolution method capable of producing images with resolutions far exceeding that of the classical diffraction limit. However, like all optical microscopes, SMS microscopes are sensitive to, and often limited by, specimen-induced aberrations. Adaptive optics (AO) has proven beneficial in a range of microscopes to overcome the limitations caused by aberrations. We report here on new AO methods for SMS microscopy that enable the feedback correction of specimen-induced aberrations. The benefits are demonstrated through two-dimensional and three-dimensional STORM imaging. We expect that this advance will broaden the scope of SMS microscopy by enabling deep-cell and tissue-level imaging.

LanguageEnglish
Pages177-185
Number of pages9
JournalOptica Applicata
Volume2
Issue number2
DOIs
Publication statusPublished - 19 Feb 2015
Externally publishedYes

Fingerprint

Adaptive optics
Aberrations
adaptive optics
aberration
Microscopic examination
microscopy
Molecules
Microscopes
molecules
microscopes
Imaging techniques
optical microscopes
Diffraction
Tissue
Feedback
cells
diffraction

Keywords

  • fluorescence microscopy
  • or adaptive optics
  • resolution
  • three-dimensional microscopy

Cite this

Burke, Daniel ; Patton, Brian ; Huang, Fang ; Bewersdorf, Joerg ; Booth, Martin J. / Adaptive optics correction of specimen-induced aberrations in single-molecule switching microscopy. In: Optica Applicata. 2015 ; Vol. 2, No. 2. pp. 177-185.
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Adaptive optics correction of specimen-induced aberrations in single-molecule switching microscopy. / Burke, Daniel; Patton, Brian; Huang, Fang; Bewersdorf, Joerg; Booth, Martin J.

In: Optica Applicata, Vol. 2, No. 2, 19.02.2015, p. 177-185.

Research output: Contribution to journalArticle

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