Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

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Abstract

We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the SuperResolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.
Original languageEnglish
Number of pages7
JournalF1000Research
Early online date26 Nov 2019
DOIs
Publication statusE-pub ahead of print - 26 Nov 2019

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Microscopes
microscopes
Imaging techniques
Costs and Cost Analysis
Optical Imaging
Costs
Diffraction
Fluorescence
fluorescence
diffraction

Keywords

  • light microscopy
  • super-resolution
  • 3D printing

Cite this

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abstract = "We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the SuperResolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.",
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