Adaptation to endophytic lifestyle through genome reduction by Kitasatospora sp. SUK42

Noraziah M. Zin, Aisha Ismail, David R. Mark, Gareth Westrop, Jana K. Schniete, Paul Herron

Research output: Contribution to journalArticlepeer-review

1 Downloads (Pure)

Abstract

Endophytic actinobacteria offer great potential as a source of novel bioactive compounds. In order to investigate the potential for the production of secondary metabolites by endophytes, we recovered a filamentous microorgansism from the tree Antidesma neurocarpum Miq. After phenotypic analysis and whole genome sequencing we demonstrated that this organism, SUK42 was a member of the actinobacterial genus Kitasatospora. This strain has a small genome in comparison with other type strains of this genus and has lost metabolic pathways associated with Stress Response, Nitrogen Metabolism and Secondary Metabolism. Despite this SUK42 can grow well in a laboratory environment and encodes a core genome that is consistent with other members of the genus. Finally, in contrast to other members of Kitasatospora, SUK42 encodes saccharide secondary metabolite biosynthetic gene clusters, one of which with similarity to the acarviostatin cluster, the product of which displays α-amylase inhibitory activity. As extracts of the host plant demonstrate this inhibitory activity, it suggests that the potential medicinal properties of A. neurocarpum Miq might be provided by the endophytic partner and illustrate the potential for exploitation of endophytes for clinical or industrial uses.
Original languageEnglish
Article number740722
Number of pages12
JournalFrontiers in Bioengineering and Biotechnology
Volume9
Early online date12 Oct 2021
DOIs
Publication statusPublished - 12 Oct 2021

Keywords

  • endophyte
  • Kitasatospora
  • genome
  • pan-genome
  • Antidesma neurocarpum miq

Fingerprint

Dive into the research topics of 'Adaptation to endophytic lifestyle through genome reduction by Kitasatospora sp. SUK42'. Together they form a unique fingerprint.

Cite this