Acute interaction between hydrocortisone and insulin alters the plasma metabolome in humans

Mohammad A. Alwashih, Roland H. Stimson, Ruth Andrew, Brian R. Walker, David G. Watson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

With the aim of identifying biomarkers of glucocorticoid action and their relationship with biomarkers of insulin action, metabolomic profiling was carried out in plasma samples from twenty healthy men who were administered either a low or medium dose insulin infusion (n = 10 each group). In addition, all subjects were given metyrapone (to inhibit adrenal cortisol secretion) +/-hydrocortisone (HC) in a randomised crossover design to produce low, medium and high glucocorticoid levels. The clearest effects of insulin were to reduce plasma levels of the branched chain amino acids (BCAs) leucine/isoleucine and their deaminated metabolites, and lowered free fatty acids and acylcarnitines. The highest dose of hydrocortisone increased plasma BCAs in both insulin groups but increased free fatty acids only in the high insulin group, however hydrocortisone did not affect the levels of acyl carnitines in either group. The clearest interaction between HC and insulin was that hydrocortisone produced an elevation in levels of BCAs and their metabolites which were lowered by insulin. The direct modulation of BCAs by glucocorticoids and insulin may provide the basis for improved in vivo monitoring of glucocorticoid and insulin action.

LanguageEnglish
Article number11488
Number of pages10
JournalScientific Reports
Volume7
Issue number1
Early online date13 Sep 2017
DOIs
Publication statusE-pub ahead of print - 13 Sep 2017

Fingerprint

Metabolome
Hydrocortisone
Insulin
Branched Chain Amino Acids
Glucocorticoids
Nonesterified Fatty Acids
Biomarkers
Metyrapone
Metabolomics
Carnitine
Isoleucine
Leucine
Cross-Over Studies

Keywords

  • hydrocortisone
  • insulin
  • biomarkers
  • glucocorticoids

Cite this

Alwashih, Mohammad A. ; Stimson, Roland H. ; Andrew, Ruth ; Walker, Brian R. ; Watson, David G. / Acute interaction between hydrocortisone and insulin alters the plasma metabolome in humans. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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Acute interaction between hydrocortisone and insulin alters the plasma metabolome in humans. / Alwashih, Mohammad A.; Stimson, Roland H.; Andrew, Ruth; Walker, Brian R.; Watson, David G.

In: Scientific Reports, Vol. 7, No. 1, 11488, 13.09.2017.

Research output: Contribution to journalArticle

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