Abstract
A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degreesC. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h.
| Original language | English |
|---|---|
| Pages (from-to) | 133-136 |
| Number of pages | 3 |
| Journal | Biotechnology Letters |
| Volume | 26 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 2004 |
Keywords
- immobilized papain
- low-water media
- propanol-rinsed enzyme preparations
- Organic-solvents
- enzyme-activity
- buffers
Fingerprint
Dive into the research topics of 'Activity of immobilized papain dehydrated by n-propanol in low- water media'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver