Activity of immobilized papain dehydrated by n-propanol in low- water media

T. Theppakorn, P. Kanasawud, P.J. Halling

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degreesC. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h.
Original languageEnglish
Pages (from-to)133-136
Number of pages3
JournalBiotechnology Letters
Issue number2
Publication statusPublished - Jan 2004


  • immobilized papain
  • low-water media
  • propanol-rinsed enzyme preparations
  • Organic-solvents
  • enzyme-activity
  • buffers


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