Activity of immobilized papain dehydrated by n-propanol in low- water media

T. Theppakorn, P. Kanasawud, P.J. Halling

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degreesC. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h.
LanguageEnglish
Pages133-136
Number of pages3
JournalBiotechnology Letters
Volume26
Issue number2
DOIs
Publication statusPublished - Jan 2004

Fingerprint

1-Propanol
Papain
tert-Butyl Alcohol
Water
Powders
Cysteine
Buffers
Salts
Enzymes
carbobenzoxyglycylphenylalanine amide

Keywords

  • immobilized papain
  • low-water media
  • propanol-rinsed enzyme preparations
  • Organic-solvents
  • enzyme-activity
  • buffers

Cite this

Theppakorn, T. ; Kanasawud, P. ; Halling, P.J. / Activity of immobilized papain dehydrated by n-propanol in low- water media. In: Biotechnology Letters. 2004 ; Vol. 26, No. 2. pp. 133-136.
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Activity of immobilized papain dehydrated by n-propanol in low- water media. / Theppakorn, T.; Kanasawud, P.; Halling, P.J.

In: Biotechnology Letters, Vol. 26, No. 2, 01.2004, p. 133-136.

Research output: Contribution to journalArticle

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KW - low-water media

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KW - Organic-solvents

KW - enzyme-activity

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