Active focus locking in an optically sectioning microscope utilizing a deformable membrane mirror

S.P. Poland, A.J. Wright, J.M. Girkin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A significant challenge for in vivo imaging is to remove movement artifacts. These movements (typically due to either respiration and cardiac-related movement or surface chemical response) are normally limited to the axial direction, and hence features move in and out of the focal plane. This presents a real problem for high resolution optically sectioned imaging techniques such as confocal and multiphoton microscopy. To overcome this we have developed an actively locked focus-tracking system based around a deformable membrane mirror. This has a significant advantage over more conventional focus-tracking techniques where the microscope objective is dithered, since the active element is not in direct, or indirect, contact with the sample. To examine the operational limits and to demonstrate possible applications for this form of focus locking, sample oscillation and movement are simulated for two different biological applications. We were able to track focus over a 400 m range (limited by the range of the piezomounted objective) with a rms precision on the focal depth of 0.31 m±0.05 m.
Original languageEnglish
Pages (from-to)419-421
Number of pages2
JournalOptics Letters
Volume33
Issue number5
DOIs
Publication statusPublished - 20 Feb 2008

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locking
microscopes
mirrors
membranes
respiration
imaging techniques
artifacts
microscopy
oscillations
high resolution

Keywords

  • Physics
  • Photonics.

Cite this

Poland, S.P. ; Wright, A.J. ; Girkin, J.M. / Active focus locking in an optically sectioning microscope utilizing a deformable membrane mirror. In: Optics Letters. 2008 ; Vol. 33, No. 5. pp. 419-421.
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Active focus locking in an optically sectioning microscope utilizing a deformable membrane mirror. / Poland, S.P.; Wright, A.J.; Girkin, J.M.

In: Optics Letters, Vol. 33, No. 5, 20.02.2008, p. 419-421.

Research output: Contribution to journalArticle

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