Achieving very low levels of detection

an improved surface-enhanced raman scattering experiment for the physical chemistry teaching laboratory

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Abstract

This experiment was designed and successfully introduced to complement the nanochemistry taught to undergraduate students in a useful and interesting way. Colloidal Ag nanoparticles were synthesized by a simple, room-temperature method, and the resulting suspension was then used to study the surface-enhanced Raman scattering (SERS) of methylene blue. The colloid was also characterized by UV–visible spectroscopy, and these results were used to help explain some of the observed SERS features. The students looked at the effects of concentration and acquisition time on the measured SERS spectra, and the final part of the experiment was based around using their newly acquired knowledge to investigate the lowest concentration of methylene blue that could be detected. Concentrations of 5 × 10-10 M were routinely achieved. The combination of UV–visible spectroscopy, SERS, and nanochemistry made for an interesting and thought-provoking laboratory experience.
Original languageEnglish
Number of pages5
JournalJournal of Chemical Education
Early online date5 Aug 2016
DOIs
Publication statusE-pub ahead of print - 5 Aug 2016

Fingerprint

Physical chemistry
Raman scattering
Teaching
chemistry
Methylene Blue
experiment
student
Experiments
Spectroscopy
Students
Colloids
Suspensions
experience
Nanoparticles
Temperature
time

Keywords

  • analytical Chemistry
  • laboratory Instruction
  • raman spectroscopy
  • physical chemistry
  • problem solving/decision making
  • nanotechnology
  • colloids
  • hands-on learning/manipulatives
  • UV-vis spectroscopy
  • upper-division undergraduate

Cite this

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title = "Achieving very low levels of detection: an improved surface-enhanced raman scattering experiment for the physical chemistry teaching laboratory",
abstract = "This experiment was designed and successfully introduced to complement the nanochemistry taught to undergraduate students in a useful and interesting way. Colloidal Ag nanoparticles were synthesized by a simple, room-temperature method, and the resulting suspension was then used to study the surface-enhanced Raman scattering (SERS) of methylene blue. The colloid was also characterized by UV–visible spectroscopy, and these results were used to help explain some of the observed SERS features. The students looked at the effects of concentration and acquisition time on the measured SERS spectra, and the final part of the experiment was based around using their newly acquired knowledge to investigate the lowest concentration of methylene blue that could be detected. Concentrations of 5 × 10-10 M were routinely achieved. The combination of UV–visible spectroscopy, SERS, and nanochemistry made for an interesting and thought-provoking laboratory experience.",
keywords = "analytical Chemistry, laboratory Instruction, raman spectroscopy, physical chemistry, problem solving/decision making, nanotechnology, colloids, hands-on learning/manipulatives, UV-vis spectroscopy, upper-division undergraduate",
author = "McMillan, {Brian G.}",
note = "This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Journal of Chemical Education, copyright {\circledC} American Chemical Society after peer review. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jchemed.6b00312",
year = "2016",
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language = "English",
journal = "Journal of Chemical Education",
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AU - McMillan, Brian G.

N1 - This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Journal of Chemical Education, copyright © American Chemical Society after peer review. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jchemed.6b00312

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Y1 - 2016/8/5

N2 - This experiment was designed and successfully introduced to complement the nanochemistry taught to undergraduate students in a useful and interesting way. Colloidal Ag nanoparticles were synthesized by a simple, room-temperature method, and the resulting suspension was then used to study the surface-enhanced Raman scattering (SERS) of methylene blue. The colloid was also characterized by UV–visible spectroscopy, and these results were used to help explain some of the observed SERS features. The students looked at the effects of concentration and acquisition time on the measured SERS spectra, and the final part of the experiment was based around using their newly acquired knowledge to investigate the lowest concentration of methylene blue that could be detected. Concentrations of 5 × 10-10 M were routinely achieved. The combination of UV–visible spectroscopy, SERS, and nanochemistry made for an interesting and thought-provoking laboratory experience.

AB - This experiment was designed and successfully introduced to complement the nanochemistry taught to undergraduate students in a useful and interesting way. Colloidal Ag nanoparticles were synthesized by a simple, room-temperature method, and the resulting suspension was then used to study the surface-enhanced Raman scattering (SERS) of methylene blue. The colloid was also characterized by UV–visible spectroscopy, and these results were used to help explain some of the observed SERS features. The students looked at the effects of concentration and acquisition time on the measured SERS spectra, and the final part of the experiment was based around using their newly acquired knowledge to investigate the lowest concentration of methylene blue that could be detected. Concentrations of 5 × 10-10 M were routinely achieved. The combination of UV–visible spectroscopy, SERS, and nanochemistry made for an interesting and thought-provoking laboratory experience.

KW - analytical Chemistry

KW - laboratory Instruction

KW - raman spectroscopy

KW - physical chemistry

KW - problem solving/decision making

KW - nanotechnology

KW - colloids

KW - hands-on learning/manipulatives

KW - UV-vis spectroscopy

KW - upper-division undergraduate

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