Absence of 5 alpha-reductase activity in the gubernaculum during descent of the fetal pig testis

C F Heyns, R Tate, N S Sargent, F K Habib, G D Chisholm

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To evaluate the hypothesis that testicular descent is mediated by the action of high local concentrations of dihydrotestosterone in the gubernaculum, testis, or epididymis, we obtained tissues from 30 male pig fetuses between 63 and 101 days of gestation. We assayed the 5 alpha-reductase activity in homogenates of pooled tissue by following the conversion of (3H)testosterone to (3H)dihydrotestosterone. The 5 alpha-reductase activity in the urethra and prostate increased prior to and during, but decreased after, the period of testicular descent. The 5 alpha-reductase activity in the gubernaculum remained constant throughout gestation and was not significantly higher than the background activity found in umbilical cord, testis plus epididymis, striated thigh muscle and heat inactivated samples of prostate and urethra. Prior to testicular descent, the 5 alpha-reductase activity was approximately 60 to 300 times higher in the urethra than in the gubernaculum, and approximately 20 to 55 times higher in the prostate than in the gubernaculum. These findings indicate that local conversion of testosterone to dihydrotestosterone in the gubernaculum or epididymis does not play a role in the mediation of testicular descent in the pig fetus.
LanguageEnglish
Pages510-513
Number of pages4
JournalJournal of Urology
Volume150
Issue number2 Pt 1
Publication statusPublished - 1993

Fingerprint

Cholestenone 5 alpha-Reductase
Testis
Swine
Dihydrotestosterone
Epididymis
Urethra
Prostate
Testosterone
Fetus
Pregnancy
Striated Muscle
Umbilical Cord
Thigh
Hot Temperature
Gubernaculum

Keywords

  • animals
  • cholestenone 5 alpha-Reductase
  • fetus
  • gestational age
  • ligaments
  • male
  • muscles
  • oxidoreductases
  • prostate
  • swine
  • testis
  • umbilical cord
  • urethra

Cite this

Heyns, C. F., Tate, R., Sargent, N. S., Habib, F. K., & Chisholm, G. D. (1993). Absence of 5 alpha-reductase activity in the gubernaculum during descent of the fetal pig testis. Journal of Urology, 150(2 Pt 1), 510-513.
Heyns, C F ; Tate, R ; Sargent, N S ; Habib, F K ; Chisholm, G D. / Absence of 5 alpha-reductase activity in the gubernaculum during descent of the fetal pig testis. In: Journal of Urology. 1993 ; Vol. 150, No. 2 Pt 1. pp. 510-513.
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Heyns, CF, Tate, R, Sargent, NS, Habib, FK & Chisholm, GD 1993, 'Absence of 5 alpha-reductase activity in the gubernaculum during descent of the fetal pig testis' Journal of Urology, vol. 150, no. 2 Pt 1, pp. 510-513.

Absence of 5 alpha-reductase activity in the gubernaculum during descent of the fetal pig testis. / Heyns, C F; Tate, R; Sargent, N S; Habib, F K; Chisholm, G D.

In: Journal of Urology, Vol. 150, No. 2 Pt 1, 1993, p. 510-513.

Research output: Contribution to journalArticle

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