Ablation of the androgen receptor from vascular smooth muscle cells demonstrates a role for testosterone in vascular calcification

Dongxing Zhu, Patrick W.F. Hadoke, Junxi Wu, Alex T. Vesey, Daniel A. Lerman, Marc R. Dweck, David E. Newby, Lee B. Smith, Vicky E. MacRae

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Vascular calcification powerfully predicts mortality and morbidity from cardiovascular disease. Men have a greater risk of cardiovascular disease, compared to women of a similar age. These gender disparities suggest an influence of sex hormones. Testosterone is the primary and most well-recognised androgen in men. Therefore, we addressed the hypothesis that exogenous androgen treatment induces vascular calcification. Immunohistochemical analysis revealed expression of androgen receptor (AR) in the calcified media of human femoral artery tissue and calcified human valves. Furthermore, in vitro studies revealed increased phosphate (Pi)-induced mouse vascular smooth muscle cell (VSMC) calcification following either testosterone or dihydrotestosterone (DHT) treatment for 9 days. Testosterone and DHT treatment increased tissue non-specific alkaline phosphatase (Alpl) mRNA expression. Testosterone-induced calcification was blunted in VSMC-specific AR-ablated (SM-ARKO) VSMCs compared to WT. Consistent with these data, SM-ARKO VSMCs showed a reduction in Osterix mRNA expression. However, intriguingly, a counter-intuitive increase in Alpl was observed. These novel data demonstrate that androgens play a role in inducing vascular calcification through the AR. Androgen signalling may represent a novel potential therapeutic target for clinical intervention.

Original languageEnglish
Article number24807
Pages (from-to)1-9
Number of pages9
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 20 Apr 2016

Keywords

  • androgen receptor
  • testosterone
  • blood vessel calcification
  • drug effects
  • apoptosis
  • vascular smooth muscle

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