A vertically integrated CMOS microsystem for time-resolved fluorescence analysis

B.R. Rae; J Yang; Jonathan Mckendry; Zheng Gong; D. Renshaw; J.M. Girkin; Erdan Gu; Martin Dawson; R.K. Henderson

doi:10.1109/TBCAS.2010.2077290

A vertically integrated CMOS microsystem for time-resolved fluorescence analysis

B.R. Rae
, J Yang
, Jonathan Mckendry
, Zheng Gong
, D. Renshaw
, J.M. Girkin
, Erdan Gu
, Martin Dawson
, R.K. Henderson

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

We describe a two-chip micro-scale time-resolved fluorescence analyzer integrating excitation, detection, and filtering. A new 8×8 array of drivers realized in standard low-voltage 0.35-μm complementary metal-oxide semiconductor is bump-bonded to AlInGaN blue micro-pixellated light-emitting diodes (micro-LEDs). The array is capable of producing sample excitation pulses with a width of 777 ps (FWHM), enabling short lifetime fluorophores to be investigated. The fluorescence emission is detected by a second, vertically-opposed 16 × 4 array of single-photon avalanche diodes (SPADs) fabricated in 0.35-μm high-voltage CMOS technology with in-pixel time-gated photon counting circuitry. Captured chip data are transferred to a PC for further processing, including histogramming, lifetime extraction, calibration and background/noise compensation. This constitutes the smallest reported solid-state microsystem for fluorescence decay analysis, replacing lasers, photomultiplier tubes, bulk optics, and discrete electronics. The system is demonstrated with measurements of fluorescent colloidal quantum dot and Rhodamine samples.

Original language	English
Pages (from-to)	437-444
Number of pages	8
Journal	IEEE Transactions on Biomedical Engineering
Volume	4
Issue number	6
DOIs	https://doi.org/10.1109/TBCAS.2010.2077290
Publication status	Published - 1 Dec 2010

Keywords

single-photon avalanche diodes
biophotonics
GaN micro light emitting diodes
biosensors
complementary metal oxide semiconductor
CMOS
integrated circuits
fluorescence
fluorescence lifetime

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10.1109/TBCAS.2010.2077290

Semiconductor-Based Hybrid Structures for Ultraviolet Micro-Devices
Dawson, M. (Principal Investigator), Calvez, S. (Co-investigator), Martin, R. (Co-investigator) & Watson, I. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/01/07 → 31/12/10
Project: Research
ADVANCED SOLID STATE LASER SOURCES AND SYSTEMS
Ferguson, A. (Principal Investigator), Burns, D. (Co-investigator), Calvez, S. (Co-investigator), Dawson, M. (Co-investigator), Girkin, J. (Co-investigator), Hastie, J. (Co-investigator) & Kemp, A. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/10/06 → 30/09/11
Project: Research

Cite this

@article{2af06fccc4ee4ee89186f385c178f5db,

title = "A vertically integrated CMOS microsystem for time-resolved fluorescence analysis",

abstract = "We describe a two-chip micro-scale time-resolved fluorescence analyzer integrating excitation, detection, and filtering. A new 8×8 array of drivers realized in standard low-voltage 0.35-μm complementary metal-oxide semiconductor is bump-bonded to AlInGaN blue micro-pixellated light-emitting diodes (micro-LEDs). The array is capable of producing sample excitation pulses with a width of 777 ps (FWHM), enabling short lifetime fluorophores to be investigated. The fluorescence emission is detected by a second, vertically-opposed 16 × 4 array of single-photon avalanche diodes (SPADs) fabricated in 0.35-μm high-voltage CMOS technology with in-pixel time-gated photon counting circuitry. Captured chip data are transferred to a PC for further processing, including histogramming, lifetime extraction, calibration and background/noise compensation. This constitutes the smallest reported solid-state microsystem for fluorescence decay analysis, replacing lasers, photomultiplier tubes, bulk optics, and discrete electronics. The system is demonstrated with measurements of fluorescent colloidal quantum dot and Rhodamine samples.",

keywords = "single-photon avalanche diodes , biophotonics, GaN micro light emitting diodes, biosensors, complementary metal oxide semiconductor , CMOS, integrated circuits, fluorescence, fluorescence lifetime",

author = "B.R. Rae and J Yang and Jonathan Mckendry and Zheng Gong and D. Renshaw and J.M. Girkin and Erdan Gu and Martin Dawson and R.K. Henderson",

year = "2010",

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doi = "10.1109/TBCAS.2010.2077290",

language = "English",

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T1 - A vertically integrated CMOS microsystem for time-resolved fluorescence analysis

AU - Rae, B.R.

AU - Yang, J

AU - Mckendry, Jonathan

AU - Gong, Zheng

AU - Renshaw, D.

AU - Girkin, J.M.

AU - Gu, Erdan

AU - Dawson, Martin

AU - Henderson, R.K.

PY - 2010/12/1

Y1 - 2010/12/1

N2 - We describe a two-chip micro-scale time-resolved fluorescence analyzer integrating excitation, detection, and filtering. A new 8×8 array of drivers realized in standard low-voltage 0.35-μm complementary metal-oxide semiconductor is bump-bonded to AlInGaN blue micro-pixellated light-emitting diodes (micro-LEDs). The array is capable of producing sample excitation pulses with a width of 777 ps (FWHM), enabling short lifetime fluorophores to be investigated. The fluorescence emission is detected by a second, vertically-opposed 16 × 4 array of single-photon avalanche diodes (SPADs) fabricated in 0.35-μm high-voltage CMOS technology with in-pixel time-gated photon counting circuitry. Captured chip data are transferred to a PC for further processing, including histogramming, lifetime extraction, calibration and background/noise compensation. This constitutes the smallest reported solid-state microsystem for fluorescence decay analysis, replacing lasers, photomultiplier tubes, bulk optics, and discrete electronics. The system is demonstrated with measurements of fluorescent colloidal quantum dot and Rhodamine samples.

AB - We describe a two-chip micro-scale time-resolved fluorescence analyzer integrating excitation, detection, and filtering. A new 8×8 array of drivers realized in standard low-voltage 0.35-μm complementary metal-oxide semiconductor is bump-bonded to AlInGaN blue micro-pixellated light-emitting diodes (micro-LEDs). The array is capable of producing sample excitation pulses with a width of 777 ps (FWHM), enabling short lifetime fluorophores to be investigated. The fluorescence emission is detected by a second, vertically-opposed 16 × 4 array of single-photon avalanche diodes (SPADs) fabricated in 0.35-μm high-voltage CMOS technology with in-pixel time-gated photon counting circuitry. Captured chip data are transferred to a PC for further processing, including histogramming, lifetime extraction, calibration and background/noise compensation. This constitutes the smallest reported solid-state microsystem for fluorescence decay analysis, replacing lasers, photomultiplier tubes, bulk optics, and discrete electronics. The system is demonstrated with measurements of fluorescent colloidal quantum dot and Rhodamine samples.

KW - single-photon avalanche diodes

KW - biophotonics

KW - GaN micro light emitting diodes

KW - biosensors

KW - complementary metal oxide semiconductor

KW - CMOS

KW - integrated circuits

KW - fluorescence

KW - fluorescence lifetime

U2 - 10.1109/TBCAS.2010.2077290

DO - 10.1109/TBCAS.2010.2077290

M3 - Article

SN - 0018-9294

VL - 4

SP - 437

EP - 444

JO - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

IS - 6

ER -

A vertically integrated CMOS microsystem for time-resolved fluorescence analysis

Abstract

Keywords

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Projects

Semiconductor-Based Hybrid Structures for Ultraviolet Micro-Devices

ADVANCED SOLID STATE LASER SOURCES AND SYSTEMS

Cite this