Abstract
| Language | English |
|---|---|
| Pages | 437-444 |
| Number of pages | 8 |
| Journal | IEEE Transactions on Biomedical Engineering |
| Volume | 4 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Dec 2010 |
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Keywords
- single-photon avalanche diodes
- biophotonics
- GaN micro light emitting diodes
- biosensors
- complementary metal oxide semiconductor
- CMOS
- integrated circuits
- fluorescence
- fluorescence lifetime
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A vertically integrated CMOS microsystem for time-resolved fluorescence analysis. / Rae, B.R.; Yang, J; Mckendry, Jonathan; Gong, Zheng; Renshaw, D.; Girkin, J.M.; Gu, Erdan; Dawson, Martin; Henderson, R.K.
In: IEEE Transactions on Biomedical Engineering, Vol. 4, No. 6, 01.12.2010, p. 437-444.Research output: Contribution to journal › Article
TY - JOUR
T1 - A vertically integrated CMOS microsystem for time-resolved fluorescence analysis
AU - Rae, B.R.
AU - Yang, J
AU - Mckendry, Jonathan
AU - Gong, Zheng
AU - Renshaw, D.
AU - Girkin, J.M.
AU - Gu, Erdan
AU - Dawson, Martin
AU - Henderson, R.K.
PY - 2010/12/1
Y1 - 2010/12/1
N2 - We describe a two-chip micro-scale time-resolved fluorescence analyzer integrating excitation, detection, and filtering. A new 8×8 array of drivers realized in standard low-voltage 0.35-μm complementary metal-oxide semiconductor is bump-bonded to AlInGaN blue micro-pixellated light-emitting diodes (micro-LEDs). The array is capable of producing sample excitation pulses with a width of 777 ps (FWHM), enabling short lifetime fluorophores to be investigated. The fluorescence emission is detected by a second, vertically-opposed 16 × 4 array of single-photon avalanche diodes (SPADs) fabricated in 0.35-μm high-voltage CMOS technology with in-pixel time-gated photon counting circuitry. Captured chip data are transferred to a PC for further processing, including histogramming, lifetime extraction, calibration and background/noise compensation. This constitutes the smallest reported solid-state microsystem for fluorescence decay analysis, replacing lasers, photomultiplier tubes, bulk optics, and discrete electronics. The system is demonstrated with measurements of fluorescent colloidal quantum dot and Rhodamine samples.
AB - We describe a two-chip micro-scale time-resolved fluorescence analyzer integrating excitation, detection, and filtering. A new 8×8 array of drivers realized in standard low-voltage 0.35-μm complementary metal-oxide semiconductor is bump-bonded to AlInGaN blue micro-pixellated light-emitting diodes (micro-LEDs). The array is capable of producing sample excitation pulses with a width of 777 ps (FWHM), enabling short lifetime fluorophores to be investigated. The fluorescence emission is detected by a second, vertically-opposed 16 × 4 array of single-photon avalanche diodes (SPADs) fabricated in 0.35-μm high-voltage CMOS technology with in-pixel time-gated photon counting circuitry. Captured chip data are transferred to a PC for further processing, including histogramming, lifetime extraction, calibration and background/noise compensation. This constitutes the smallest reported solid-state microsystem for fluorescence decay analysis, replacing lasers, photomultiplier tubes, bulk optics, and discrete electronics. The system is demonstrated with measurements of fluorescent colloidal quantum dot and Rhodamine samples.
KW - single-photon avalanche diodes
KW - biophotonics
KW - GaN micro light emitting diodes
KW - biosensors
KW - complementary metal oxide semiconductor
KW - CMOS
KW - integrated circuits
KW - fluorescence
KW - fluorescence lifetime
U2 - 10.1109/TBCAS.2010.2077290
DO - 10.1109/TBCAS.2010.2077290
M3 - Article
VL - 4
SP - 437
EP - 444
JO - IEEE Transactions on Biomedical Engineering
T2 - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
SN - 0018-9294
IS - 6
ER -