A validated and stability indicating HPLC method for analysis of diminazene aceturate and antipyrine combination in a ready injectable solution

M.N. Abualhasan, N. Batrawi, A.N. Zaid, D.G. Watson

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2 Citations (Scopus)

Abstract

Diminazene aceturate and Antipyrine combination therapy is widely used in veterinary medicine. A simple reverse HPLC method for the analysis of samples of a ready injectable formulation containing a mixture of active ingredients and inactive excipients has been developed. The HPLC analysis was carried out using a reversed phase (RP)-C18 (250 mm×4.0 mm, 5 μm) column. The isocratic mobile phase consisted of a mixture of acetonitrile, methanol, phosphate buffer and hexane sulfonate; the flow rate was 0.6 mL/min and ultraviolet detection was at 291 nm. This method was validated in accordance with FDA and ICH guidelines and showed good linearity, accuracy, precision, selectivity and the system suitability results were within the acceptance criteria. A stability-indicating study was also carried out and indicated that this method could be used for purity and degradation evaluation of these formulations.
Original languageEnglish
Pages (from-to)300-304
Number of pages5
JournalDrug Research
Volume63
Issue number6
DOIs
Publication statusPublished - 1 Jun 2013

Keywords

  • HPLC method
  • analysis
  • ready injectable solution
  • diminazene aceturate
  • antipyrine combination

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