TY - JOUR
T1 - A transposon insertion single-gene knockout library and new ordered cosmid library for the model organism Streptomyces coelicolor A3(2)
AU - Fernández-Martínez, L T
AU - Del Sol, R.
AU - Evans, M.C.
AU - Fielding, S.
AU - Herron, P R
AU - Chandra, G.
AU - Dyson, P.J.
PY - 2011/3
Y1 - 2011/3
N2 - A simple and high-throughput transposon mediated mutagenesis system employing in vitro shuttle transposon mutagenesis has been used to systematically mutagenise the Streptomyces coelicolor genome. To achieve the highest coverage, a new ordered cosmid library was also constructed. Individual cosmids from both the existing and new libraries were disrupted using the Tn5-based mini-transposon Tn5062. A total of 35,358 insertions were sequenced resulting in the disruption of 6,482 genes (83% of the predicted open reading frames). Complete information for both the newly generated cosmids as well as all the insertions has been uploaded onto a central database, StrepDB ( http://strepdb.streptomyces.org.uk/ ). All insertions, new cosmids and a range of transposon exchange cassettes are available for study of individual gene function.
AB - A simple and high-throughput transposon mediated mutagenesis system employing in vitro shuttle transposon mutagenesis has been used to systematically mutagenise the Streptomyces coelicolor genome. To achieve the highest coverage, a new ordered cosmid library was also constructed. Individual cosmids from both the existing and new libraries were disrupted using the Tn5-based mini-transposon Tn5062. A total of 35,358 insertions were sequenced resulting in the disruption of 6,482 genes (83% of the predicted open reading frames). Complete information for both the newly generated cosmids as well as all the insertions has been uploaded onto a central database, StrepDB ( http://strepdb.streptomyces.org.uk/ ). All insertions, new cosmids and a range of transposon exchange cassettes are available for study of individual gene function.
KW - cosmids
KW - DNA transposable elements
KW - mutagenesis, insertional
KW - streptomyces coelicolor
U2 - 10.1007/s10482-010-9518-1
DO - 10.1007/s10482-010-9518-1
M3 - Article
C2 - 20945092
SN - 0003-6072
VL - 99
SP - 515
EP - 522
JO - Antonie van Leeuwenhoek: International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek: International Journal of General and Molecular Microbiology
IS - 3
ER -