A tandem enzymatic sp2-C-methylation process: coupling in situ S-adenosyl-L-methionine formation with methyl transfer

Joanna C Sadler, Luke D Humphreys, Glenn Burley, Radka Snajdrova

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Abstract

A one-pot, two-step biocatalytic platform for the regiospecfic C-methylation and C-ethylation of aromatic substrates is described. The tandem process utilizes SalL (Salinospora tropica) for in situ synthesis of S-adenosyl-L-methionine (SAM), followed by alkylation of aromatic substrates using the C-methyltransferase NovO (Streptomyces spheroides). Application of this methodology is demonstrated by the regiospecific labelling of aromatic substrates via the transfer of methyl, ethyl and isotopically-labelled 13CH3, 13CD3 and CD3 groups from their corresponding SAM analogues formed in situ.
Original languageEnglish
JournalChembiochem
Early online date31 Mar 2017
DOIs
Publication statusE-pub ahead of print - 31 Mar 2017

Keywords

  • methyltransferase
  • alkylation
  • biocatalytic
  • S-adenosylmethionine
  • multi-enzyme reaction

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