A one-pot, two-step biocatalytic platform for the regiospecfic C-methylation and C-ethylation of aromatic substrates is described. The tandem process utilizes SalL (Salinospora tropica) for in situ synthesis of S-adenosyl-L-methionine (SAM), followed by alkylation of aromatic substrates using the C-methyltransferase NovO (Streptomyces spheroides). Application of this methodology is demonstrated by the regiospecific labelling of aromatic substrates via the transfer of methyl, ethyl and isotopically-labelled 13CH3, 13CD3 and CD3 groups from their corresponding SAM analogues formed in situ.
- multi-enzyme reaction
Sadler, J. C., Humphreys, L. D., Burley, G., & Snajdrova, R. (2017). A tandem enzymatic sp2-C-methylation process: coupling in situ S-adenosyl-L-methionine formation with methyl transfer. Chembiochem. https://doi.org/10.1002/cbic.201700115