A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro

G. Baillie, M. Owens, G.W. Halbert

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application.
Original languageEnglish
Pages (from-to)69-73
Number of pages4
JournalJournal of Lipid Research
Volume43
Publication statusPublished - Jan 2002

Keywords

  • U937
  • low density lipoprotein
  • amphipathic peptide
  • apoB
  • synthetic LDL
  • phosphate-buffered saline
  • dimethyl sulfoxide

Fingerprint Dive into the research topics of 'A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro'. Together they form a unique fingerprint.

  • Cite this