Abstract
A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application.
Original language | English |
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Pages (from-to) | 69-73 |
Number of pages | 4 |
Journal | Journal of Lipid Research |
Volume | 43 |
Publication status | Published - Jan 2002 |
Keywords
- U937
- low density lipoprotein
- amphipathic peptide
- apoB
- synthetic LDL
- phosphate-buffered saline
- dimethyl sulfoxide