A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro

G. Baillie, M. Owens, G.W. Halbert

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application.
LanguageEnglish
Pages69-73
Number of pages4
JournalJournal of Lipid Research
Volume43
Publication statusPublished - Jan 2002

Fingerprint

LDL Lipoproteins
LDL Receptors
Peptides
oxidized low density lipoprotein
In Vitro Techniques
Microemulsions
Cell proliferation
Apolipoproteins B
Assays
Cell Proliferation
Lipids
Antibodies
Research

Keywords

  • U937
  • low density lipoprotein
  • amphipathic peptide
  • apoB
  • synthetic LDL
  • phosphate-buffered saline
  • dimethyl sulfoxide

Cite this

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A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro. / Baillie, G.; Owens, M.; Halbert, G.W.

In: Journal of Lipid Research, Vol. 43, 01.2002, p. 69-73.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro

AU - Baillie, G.

AU - Owens, M.

AU - Halbert, G.W.

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AB - A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application.

KW - U937

KW - low density lipoprotein

KW - amphipathic peptide

KW - apoB

KW - synthetic LDL

KW - phosphate-buffered saline

KW - dimethyl sulfoxide

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