A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles

Yinan Zhang, Yu Chen, Jun Yu, David JS Birch

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In this report we have demonstrated a fluorescence resonant energy transfer (FRET)-fluorescence lifetime imaging microscopy (FLIM) combined approach to study the intracellular pathway of gold nanoparticles. The detected energy transfer between gold nanorods (GNRs) and green fluorescence protein (GFP) labeled Hela cell earlyendosomes and the in-depth lifetime distribution analysis on the transfer process suggest an endocytotic uptake process of GNRs. Furthermore, the FRET-FLIM method profits from a surface plasmon enhanced energy transfer mechanism when taking into consideration of GNRs and two photon excitation, and is effective in biological imaging, sensing, and even in single molecular tracing in both in vivo and in vitro studies.
Original languageEnglish
Pages (from-to)78-82
Number of pages5
JournalMedical Devices and Diagnostic Engineering
Issue number1
Publication statusPublished - 29 May 2017


  • cell imaging
  • FRET
  • gold nanoparticles
  • optical imaging


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