A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles

Research output: Contribution to journalArticle

Abstract

In this report we have demonstrated a fluorescence resonant energy transfer (FRET)-fluorescence lifetime imaging microscopy (FLIM) combined approach to study the intracellular pathway of gold nanoparticles. The detected energy transfer between gold nanorods (GNRs) and green fluorescence protein (GFP) labeled Hela cell earlyendosomes and the in-depth lifetime distribution analysis on the transfer process suggest an endocytotic uptake process of GNRs. Furthermore, the FRET-FLIM method profits from a surface plasmon enhanced energy transfer mechanism when taking into consideration of GNRs and two photon excitation, and is effective in biological imaging, sensing, and even in single molecular tracing in both in vivo and in vitro studies.
LanguageEnglish
Pages78-82
Number of pages5
JournalMedical Devices and Diagnostic Engineering
Volume2
Issue number1
DOIs
Publication statusPublished - 29 May 2017

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energy transfer
microscopy
life (durability)
fluorescence
nanoparticles
gold
nanorods
tracing
proteins
photons
cells
excitation

Keywords

  • cell imaging
  • FRET
  • gold nanoparticles
  • optical imaging

Cite this

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title = "A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles",
abstract = "In this report we have demonstrated a fluorescence resonant energy transfer (FRET)-fluorescence lifetime imaging microscopy (FLIM) combined approach to study the intracellular pathway of gold nanoparticles. The detected energy transfer between gold nanorods (GNRs) and green fluorescence protein (GFP) labeled Hela cell earlyendosomes and the in-depth lifetime distribution analysis on the transfer process suggest an endocytotic uptake process of GNRs. Furthermore, the FRET-FLIM method profits from a surface plasmon enhanced energy transfer mechanism when taking into consideration of GNRs and two photon excitation, and is effective in biological imaging, sensing, and even in single molecular tracing in both in vivo and in vitro studies.",
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author = "Yinan Zhang and Yu Chen and Jun Yu and Birch, {David JS}",
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A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles. / Zhang, Yinan; Chen, Yu; Yu, Jun; Birch, David JS.

In: Medical Devices and Diagnostic Engineering, Vol. 2, No. 1, 29.05.2017, p. 78-82.

Research output: Contribution to journalArticle

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AU - Yu, Jun

AU - Birch, David JS

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KW - cell imaging

KW - FRET

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